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Medial habenular axons form a core packet whereas lateral habenular axons course in a surrounding shell. Both groups of fibers share the same initial pathway but differ in the final segment of the tract, supposedly regulated by surface molecules. The gene <jats:italic>Amigo2<\/jats:italic> codes for a membrane adhesion molecule with an immunoglobulin\u2010like domain 2 and is selectively expressed in the medial habenula. We present it as a candidate for controlling the fasciculation behavior of medial habenula axons.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>First, we studied the development of the habenular efferents in an <jats:italic>Amigo2<\/jats:italic> lack of function mouse model. The fasciculus retroflexus showed a variable defasciculation phenotype. Gain of function experiments allowed us to generate a more condensed tract and rescued the <jats:italic>Amigo2<\/jats:italic> knock\u2010out phenotype. Changes in <jats:italic>Amigo2<\/jats:italic> function did not alter the course of habenular fibers.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>We have demonstrated that <jats:italic>Amigo2<\/jats:italic> plays a subtle role in the fasciculation of the fasciculus retroflexus.<\/jats:p><\/jats:sec>","DOI":"10.1002\/dvdy.513","type":"journal-article","created":{"date-parts":[[2022,6,21]],"date-time":"2022-06-21T10:54:05Z","timestamp":1655808845000},"page":"1834-1847","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Adhesion molecule <i>Amigo2<\/i> is involved in the fasciculation process of the fasciculus retroflexus"],"prefix":"10.1002","volume":"251","author":[{"given":"Ver\u00f3nica","family":"Company","sequence":"first","affiliation":[{"name":"Instituto de Neurociencias Universidad Miguel Hern\u00e1ndez de Elche\u2010CSIC  Sant Joan d'Alacant Alicante 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