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Phosphorylation of p120 catenin changes the nature of its activity in vitro but is virtually unexplored in embryos. We used our previously developed antisense RNA splice\u2010site morpholino targeted to endogenous p120 catenin\u2010\u03b41 to cause defects in axis elongation probing the functions of three p120 catenin tyrosine\u2010phosphorylation sites in gastrulating zebrafish embryos.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The morpholino\u2010induced defects were rescued by co\u2010injections with mouse p120 catenin\u2010\u03b41\u20103A mRNAs mutated at residues Y228 and Y217 to a non\u2010phosphorylatable phenylalanine (F) or mutated at residue Y335 to a phosphomimetic glutamic acid (E). Co\u2010injection of the complementary mutations Y228E, Y217E, or Y335F mRNAs partially rescued embryos whereas dual mutation to Y228E\u2010Y217E blocked rescue. Immunopurification showed Y228F mutant proteins preferentially interacted with Rac1, potentially promoting cell migration. In contrast, the phosphomimetic Y228E preferentially interacted with E\u2010cadherin increasing adhesion. Both Y228F and Y335F strongly bind VAV2.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>p120 catenin serves dual roles during gastrulation of zebrafish. Phosphorylation and dephosphorylation of tyrosine residues Y217, Y228, and Y335 precisely balance cell adhesion and cell migration to facilitate somite compaction and axis elongation.<\/jats:p><\/jats:sec>","DOI":"10.1002\/dvdy.524","type":"journal-article","created":{"date-parts":[[2022,8,23]],"date-time":"2022-08-23T00:23:03Z","timestamp":1661214183000},"page":"1934-1951","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Dephosphorylation of <scp>Y228<\/scp> and <scp>Y217<\/scp> and phosphorylation of <scp>Y335<\/scp> in p120 catenin activate convergent extension during zebrafish gastrulation"],"prefix":"10.1002","volume":"251","author":[{"given":"Botao","family":"Shan","sequence":"first","affiliation":[{"name":"Department of Biology University of Washington  Seattle Washington USA"},{"name":"Tulane University School of Medicine  New Orleans LA 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