{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T18:41:20Z","timestamp":1760380880721,"version":"build-2065373602"},"reference-count":53,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2023,1,17]],"date-time":"2023-01-17T00:00:00Z","timestamp":1673913600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["R01\u2010HL137364"],"award-info":[{"award-number":["R01\u2010HL137364"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["anatomypubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Developmental Dynamics"],"published-print":{"date-parts":[[2023,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p><jats:italic>Hox<\/jats:italic> genes encode transcription factors that are important for establishing the body plan. <jats:italic>Hoxa5<\/jats:italic> is a member of the mammalian <jats:italic>Hox5<\/jats:italic> paralogous group that regulates the patterning and morphology of the cervical\u2010thoracic region of the axial skeleton. <jats:italic>Hoxa5<\/jats:italic> also plays crucial functions in lung morphogenesis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We generated a <jats:italic>Hoxa5eGFP<\/jats:italic> reporter mouse line using CRISPR technology, allowing real\u2010time visualization of <jats:italic>Hoxa5<\/jats:italic> expression. Hoxa5eGFP recapitulates reported embryonic <jats:italic>Hoxa5<\/jats:italic> mRNA expression patterns. Specifically, Hoxa5eGFP can be visualized in the developing mouse neural tube, somites, lung, diaphragm, foregut, and midgut, among other organs. In the stomach, posteriorly biased Hoxa5eGFP expression correlates with a drastic morphological reduction of the corpus in <jats:italic>Hox5<\/jats:italic> paralogous mutants. Expression of Hoxa5eGFP in the lung continues in all lung fibroblast populations through postnatal and adult stages.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>We identified cell types that express <jats:italic>Hoxa5<\/jats:italic> in postnatal and adult mouse lungs, including various fibroblasts and vascular endothelial cells. This reporter line will be a powerful tool for studies of the function of <jats:italic>Hoxa5<\/jats:italic> during mouse development, homeostasis, and disease processes.<\/jats:p><\/jats:sec>","DOI":"10.1002\/dvdy.563","type":"journal-article","created":{"date-parts":[[2022,12,28]],"date-time":"2022-12-28T22:40:40Z","timestamp":1672267240000},"page":"536-546","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Characterization of a novel <scp><i>Hoxa5eGFP<\/i><\/scp> mouse line"],"prefix":"10.1002","volume":"252","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3176-5631","authenticated-orcid":false,"given":"Mu\u2010Hang","family":"Li","sequence":"first","affiliation":[{"name":"Genetics Training Program University of Wisconsin\u2010Madison  Madison Wisconsin USA"},{"name":"Department of Cell and Regenerative Biology, School of Medicine and Public Health University of Wisconsin\u2010Madison  Madison Wisconsin 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