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Previous studies demonstrate essential roles for stromal \u03b2\u2010catenin in kidney development. However, how stromal \u03b2\u2010catenin regulates kidney development is not known. We hypothesize that stromal \u03b2\u2010catenin modulates pathways and genes that facilitate communications with neighboring cell populations to regulate kidney development.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We isolated purified stromal cells with wild type, deficient, and overexpressed \u03b2\u2010catenin by fluorescence\u2010activated cell sorting and conducted RNA Sequencing. A Gene Ontology network analysis demonstrated that stromal \u03b2\u2010catenin modulates key kidney developmental processes, including branching morphogenesis, nephrogenesis and vascular formation. Specific stromal \u03b2\u2010catenin candidate target genes that may mediate these effects included secreted, cell\u2010surface and transcriptional factors that regulate branching morphogenesis and nephrogenesis (<jats:italic>Wnts<\/jats:italic>, <jats:italic>Bmp<\/jats:italic>, <jats:italic>Fgfr<\/jats:italic>, <jats:italic>Tcf\/Lef<\/jats:italic>) and secreted vascular guidance cues (<jats:italic>Angpt1<\/jats:italic>, <jats:italic>VEGF<\/jats:italic>, <jats:italic>Sema3a<\/jats:italic>). We validated established \u03b2\u2010catenin targets including <jats:italic>Lef1<\/jats:italic> and novel candidate \u03b2\u2010catenin targets including <jats:italic>Sema3e<\/jats:italic> which have unknown roles in kidney development.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These studies advance our understanding of gene and biological pathway dysregulation in the context of stromal \u03b2\u2010catenin misexpression during kidney development. Our findings suggest that during normal kidney development, stromal \u03b2\u2010catenin may regulate secreted and cell\u2010surface proteins to communicate with adjacent cell populations.<\/jats:p><\/jats:sec>","DOI":"10.1002\/dvdy.603","type":"journal-article","created":{"date-parts":[[2023,5,25]],"date-time":"2023-05-25T08:37:29Z","timestamp":1685003849000},"page":"1224-1239","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["\u03b2\u2010Catenin in the kidney stroma modulates pathways and genes to regulate kidney development"],"prefix":"10.1002","volume":"252","author":[{"given":"Erin","family":"Deacon","sequence":"first","affiliation":[{"name":"Department of Pathology and Molecular Medicine McMaster University  Hamilton Ontario Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Pathology and Molecular Medicine 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