{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,5]],"date-time":"2026-02-05T06:28:18Z","timestamp":1770272898503,"version":"3.49.0"},"reference-count":32,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2023,8,6]],"date-time":"2023-08-06T00:00:00Z","timestamp":1691280000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000055","name":"National Institute on Deafness and Other Communication Disorders","doi-asserted-by":"publisher","award":["RO1 DC013072"],"award-info":[{"award-number":["RO1 DC013072"]}],"id":[{"id":"10.13039\/100000055","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["anatomypubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Developmental Dynamics"],"published-print":{"date-parts":[[2023,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>FOXI3 is a forkhead family transcription factor that is expressed in the progenitors of craniofacial placodes, epidermal placodes, and the ectoderm and endoderm of the pharyngeal arch region. Loss of <jats:italic>Foxi3<\/jats:italic> in mice and pathogenic <jats:italic>Foxi3<\/jats:italic> variants in dogs and humans cause a variety of craniofacial defects including absence of the inner ear, severe truncations of the jaw, loss or reduction in external and middle ear structures, and defects in teeth and hair.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>To allow for the identification, isolation, and lineage tracing of Foxi3\u2010expressing cells in developing mice, we targeted the <jats:italic>Foxi3<\/jats:italic> locus to create <jats:italic>Foxi3<\/jats:italic><jats:sup><jats:italic>GFP<\/jats:italic><\/jats:sup> and <jats:italic>Foxi3<\/jats:italic><jats:sup><jats:italic>CreER<\/jats:italic><\/jats:sup> mice. We show that <jats:italic>Foxi3<\/jats:italic><jats:sup><jats:italic>GFP<\/jats:italic><\/jats:sup> mice faithfully recapitulate the expression pattern of <jats:italic>Foxi3<\/jats:italic> mRNA at all ages examined, and <jats:italic>Foxi3<\/jats:italic><jats:sup><jats:italic>CreER<\/jats:italic><\/jats:sup> mice can trace the derivatives of pharyngeal arch ectoderm and endoderm, the pharyngeal pouches and clefts that separate each arch, and the derivatives of hair and tooth placodes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p><jats:italic>Foxi3<\/jats:italic><jats:sup><jats:italic>GFP<\/jats:italic><\/jats:sup> and <jats:italic>Foxi3<\/jats:italic><jats:sup><jats:italic>CreER<\/jats:italic><\/jats:sup> mice are new tools that will be of use in identifying and manipulating pharyngeal arch ectoderm and endoderm and hair and tooth placodes.<\/jats:p><\/jats:sec>","DOI":"10.1002\/dvdy.645","type":"journal-article","created":{"date-parts":[[2023,8,6]],"date-time":"2023-08-06T13:51:05Z","timestamp":1691329865000},"page":"1462-1470","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["<scp><i>Foxi3<\/i><sup><i>GFP<\/i><\/sup><\/scp> and <scp><i>Foxi3<\/i><sup><i>CreER<\/i><\/sup><\/scp> mice allow identification and lineage labeling of pharyngeal arch ectoderm and endoderm, and tooth and hair placodes"],"prefix":"10.1002","volume":"252","author":[{"given":"Harinarayana","family":"Ankamreddy","sequence":"first","affiliation":[{"name":"Department of Neuroscience Baylor College of Medicine  Houston Texas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ankita","family":"Thawani","sequence":"additional","affiliation":[{"name":"Department of Neuroscience Baylor College of Medicine  Houston Texas 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