{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,14]],"date-time":"2025-10-14T00:31:10Z","timestamp":1760401870477,"version":"build-2065373602"},"reference-count":68,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2024,2,10]],"date-time":"2024-02-10T00:00:00Z","timestamp":1707523200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["anatomypubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Developmental Dynamics"],"published-print":{"date-parts":[[2024,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>During neurogenesis, growing axons must navigate through the complex extracellular environment and make correct synaptic connections for the proper functioning of neural circuits. The mechanisms underlying the formation of functional neural networks are still only partially understood.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Here we analyzed the role of a novel gene <jats:italic>si:ch73\u2010364h19.1\/drish<\/jats:italic> in the neural and vascular development of zebrafish embryos. We show that <jats:italic>drish<\/jats:italic> mRNA is expressed broadly and dynamically in multiple cell types including neural, glial, retinal progenitor and vascular endothelial cells throughout the early stages of embryonic development. To study Drish function during embryogenesis, we generated <jats:italic>drish<\/jats:italic> genetic mutant using CRISPR\/Cas9 genome editing. <jats:italic>drish<\/jats:italic> loss\u2010of\u2010function mutant larvae displayed defects in early retinal ganglion cell, optic nerve and the retinal inner nuclear layer formation, as well as ectopic motor axon branching. In addition, <jats:italic>drish<\/jats:italic> mutant adults exhibited deficient retinal outer nuclear layer and showed defective light response and locomotory behavior. However, vascular patterning and blood circulation were not significantly affected.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Together, these data demonstrate important roles of zebrafish <jats:italic>drish<\/jats:italic> in the retinal ganglion cell, optic nerve and interneuron development and in spinal motor axon branching.<\/jats:p><\/jats:sec>","DOI":"10.1002\/dvdy.694","type":"journal-article","created":{"date-parts":[[2024,2,10]],"date-time":"2024-02-10T06:49:06Z","timestamp":1707547746000},"page":"750-770","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Requirement of a novel gene, <i>drish<\/i>, in the zebrafish retinal ganglion cell and primary motor axon development"],"prefix":"10.1002","volume":"253","author":[{"given":"Suman","family":"Gurung","sequence":"first","affiliation":[{"name":"Department of Pathology and Cell Biology USF Health Heart Institute, University of South Florida  Tampa Florida USA"},{"name":"Division of Developmental Biology Cincinnati Children's Hospital Medical Center  Cincinnati Ohio USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicole K.","family":"Restrepo","sequence":"additional","affiliation":[{"name":"Department of Pathology and Cell Biology USF Health Heart Institute, University of South Florida  Tampa Florida USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Surendra Kumar","family":"Anand","sequence":"additional","affiliation":[{"name":"Department of Pathology and Cell Biology USF Health Heart Institute, University of South Florida  Tampa Florida USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vinoth","family":"Sittaramane","sequence":"additional","affiliation":[{"name":"Department of Biology Georgia Southern University  Statesboro Georgia USA"},{"name":"Department of Molecular and Cellular Biology Sam Houston State University College of Osteopathic Medicine  Conroe Texas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8605-6122","authenticated-orcid":false,"given":"Saulius","family":"Sumanas","sequence":"additional","affiliation":[{"name":"Department of Pathology and Cell Biology USF Health Heart Institute, University of South Florida  Tampa Florida USA"},{"name":"Division of Developmental Biology Cincinnati Children's Hospital Medical Center  Cincinnati Ohio USA"},{"name":"University of Cincinnati College of Medicine Department of Pediatrics  Cincinnati Ohio USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,2,10]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1708527"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0301\u20100082(03)00052\u20102"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.06\u201008\u201002267.1986"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.902360411"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1988.sp017239"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1995.73.1.399"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.06\u201008\u201002278.1986"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ne.17.030194.000245"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn0901\u2010877"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.conb.2019.05.008"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev\u2010neuro\u2010072116\u2010031607"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.010751"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3951\u201007.2007"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mod.2005.12.001"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.128.6.943"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.23\u201004\u201001360.2003"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.conb.2004.01.002"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.01934"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2007.01.116"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.4938\u201004.2005"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.01041"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.30388"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.289.5487.2137"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0012\u20101606(03)00121\u20100"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2003.08.005"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1421535112"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0896\u20106273(01)00312\u20109"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1186\/1749\u20108104\u20108\u20103"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0166\u20102236(96)40005\u20104"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2010.02.039"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.3389\/fncir.2021.690475"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076\u20106879(08)02804\u20108"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.130"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587\u2010021\u201001156\u20103"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.2000.4315"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1101\/2022.04.08.487609"},{"key":"e_1_2_6_38_1","unstructured":"ThisseB PflumioS F\u00fcrthauerM et al.Expression of the zebrafish genome during embryogenesis. ZFIN Direct Data Submission.2001http:\/\/zfinorg"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.45241"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood\u20102007\u201009\u2010110569"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.0040010"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2006.10.030"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2023.11.001"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.2501"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1308335110"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586\u2010019\u20101064\u2010z"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.123.1.427"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.08-12-04513.1988"},{"key":"e_1_2_6_49_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097\u20104695(199601)29:1<65::AID\u2010NEU5>3.0.CO;2\u20105"},{"key":"e_1_2_6_50_1","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1001990102"},{"key":"e_1_2_6_51_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1997.8761"},{"key":"e_1_2_6_52_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.01714"},{"key":"e_1_2_6_53_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.17-20-07796.1997"},{"key":"e_1_2_6_54_1","doi-asserted-by":"publisher","DOI":"10.1038\/320269a0"},{"key":"e_1_2_6_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mod.2018.05.005"},{"key":"e_1_2_6_56_1","doi-asserted-by":"publisher","DOI":"10.1177\/1073858410385870"},{"issue":"2","key":"e_1_2_6_57_1","first-page":"367","article-title":"Developmental origin and early differentiation of retinal Muller cells in mice","volume":"120","author":"Bhattacharjee J","year":"1975","journal-title":"J Anat"},{"key":"e_1_2_6_58_1","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1002030302"},{"key":"e_1_2_6_59_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.2002.0711"},{"key":"e_1_2_6_60_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.02087"},{"key":"e_1_2_6_61_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gku410"},{"key":"e_1_2_6_62_1","doi-asserted-by":"publisher","DOI":"10.1016\/bs.mcb.2016.01.008"},{"key":"e_1_2_6_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0091\u2010679x(08)61821\u2010x"},{"key":"e_1_2_6_64_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0960\u20109822(95)00185\u20100"},{"key":"e_1_2_6_65_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2017.03.005"},{"key":"e_1_2_6_66_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2020.06.007"},{"key":"e_1_2_6_67_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2014.09.083"},{"key":"e_1_2_6_68_1","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2000.0692"},{"key":"e_1_2_6_69_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkac240"}],"container-title":["Developmental Dynamics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dvdy.694","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T12:07:24Z","timestamp":1760357244000},"score":1,"resource":{"primary":{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/10.1002\/dvdy.694"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,2,10]]},"references-count":68,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2024,8]]}},"alternative-id":["10.1002\/dvdy.694"],"URL":"https:\/\/doi.org\/10.1002\/dvdy.694","archive":["Portico"],"relation":{},"ISSN":["1058-8388","1097-0177"],"issn-type":[{"type":"print","value":"1058-8388"},{"type":"electronic","value":"1097-0177"}],"subject":[],"published":{"date-parts":[[2024,2,10]]},"assertion":[{"value":"2023-02-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-01-13","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-02-10","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}