{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T20:02:06Z","timestamp":1791316926631,"version":"4.3.1"},"reference-count":45,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,3,26]],"date-time":"2025-03-26T00:00:00Z","timestamp":1742947200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["T32\u2010GM132055"],"award-info":[{"award-number":["T32\u2010GM132055"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["R01HL122906"],"award-info":[{"award-number":["R01HL122906"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["R01HL162913"],"award-info":[{"award-number":["R01HL162913"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["T32\u2010HL007260"],"award-info":[{"award-number":["T32\u2010HL007260"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000968","name":"American Heart Association","doi-asserted-by":"publisher","award":["PRE\u20101014420"],"award-info":[{"award-number":["PRE\u20101014420"]}],"id":[{"id":"10.13039\/100000968","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["anatomypubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Developmental Dynamics"],"published-print":{"date-parts":[[2025,12]]},"abstract":"Abstract<\/jats:title>\n \n Background<\/jats:title>\n \n Previously, we explored the role of\n Sox9<\/jats:italic>\n in the second heart field (SHF) in atrioventricular septation. For that study, we created a SHF\u2010specific\n Sox9<\/jats:italic>\n knockout mouse. In addition to the presence of primary atrial septal defects in half of the offspring, we found that virtually all specimens also developed a ventricular septal defect. Histological analysis suggested that the ventricular septal defects resulted from developmental perturbation of the mesenchymal structures within the outflow tract. In the current study, we investigated the role of\n Sox9<\/jats:italic>\n in the SHF in the development of these tissues.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Sox9<\/jats:italic>\n is expressed in all mesenchymal cell populations in the developing outflow tract, including a cohort of endocardial\u2010derived cells that originate from the SHF\u2010derived endocardium. SHF\u2010specific deletion of\n Sox9<\/jats:italic>\n inhibits the formation of this cell population and ultimately leads to truncation of the mesenchymal outlet septum. This prevents complete fusion of this outlet septum with the atrioventricular mesenchymal complex, resulting in ventricular septal defects.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n \n In combination with our first paper on the role of\n Sox9<\/jats:italic>\n in atrioventricular septation, data presented in this study demonstrate that\n Sox9<\/jats:italic>\n expression in the SHF is of critical importance for the proper formation of the septal structures in the developing heart.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/dvdy.70014","type":"journal-article","created":{"date-parts":[[2025,3,29]],"date-time":"2025-03-29T04:34:16Z","timestamp":1743222856000},"page":"1325-1344","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Sox9<\/i>\n in the second heart field and the development of the outflow tract; implications for cardiac septation and valve formation"],"prefix":"10.1002","volume":"254","author":[{"given":"Jenna R.","family":"Drummond","sequence":"first","affiliation":[{"name":"Department of Regenerative Medicine and Cell Biology Medical University of South Carolina Charleston South Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raymond N.","family":"Deepe","sequence":"additional","affiliation":[{"name":"Department of Regenerative Medicine and Cell Biology Medical University of South Carolina Charleston South Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hannah G.","family":"Tarolli","sequence":"additional","affiliation":[{"name":"Department of Regenerative Medicine and Cell Biology Medical University of South Carolina Charleston South Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ren\u00e9lyn A.","family":"Wolters","sequence":"additional","affiliation":[{"name":"Department of Regenerative Medicine and Cell Biology Medical 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