{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T21:24:40Z","timestamp":1789248280836,"version":"build-2803163510"},"reference-count":45,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2025,12,24]],"date-time":"2025-12-24T00:00:00Z","timestamp":1766534400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,12,24]],"date-time":"2025-12-24T00:00:00Z","timestamp":1766534400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["anatomypubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Developmental Dynamics"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Mycn, a MYC gene family member, is implicated in both carcinogenesis through amplification and Feingold syndrome through its deficiency. Previous studies have indicated that increased Mycn expression enhances vascularization in human neuroblastomas, yet its precise role in vascular development remains elusive.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      In this study, we utilized single\u2010cell RNA\u2010seq and live imaging analyses to confirm that\n                      <jats:italic>mycn<\/jats:italic>\n                      is expressed during zebrafish vasculogenesis. We investigated vascular development in zebrafish using a genetically engineered\n                      <jats:italic>mycn<\/jats:italic>\n                      mutation. Our findings reveal that\n                      <jats:italic>mycn<\/jats:italic>\n                      \u2010deficient zebrafish exhibit reduced intersegmental vessels and malformed subintestinal vessels, primarily due to decreased cell proliferation in vascular endothelial cells. Importantly, we discovered that activation of PI3K signaling significantly ameliorates these vascular abnormalities.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Our study establishes Mycn as a key regulator of vascular development in zebrafish, acting through the PI3K signaling pathway.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/dvdy.70104","type":"journal-article","created":{"date-parts":[[2025,12,24]],"date-time":"2025-12-24T10:42:33Z","timestamp":1766572953000},"page":"881-895","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Mycn regulates vascular development through\n                    <scp>PI3K<\/scp>\n                    signaling pathway in zebrafish"],"prefix":"10.1002","volume":"255","author":[{"given":"Guo\u2010Qin","family":"Zhao","sequence":"first","affiliation":[{"name":"Department of Immunology, National and Guizhou Joint Engineering Laboratory for Cell Engineering and Biomedicine Technique, School of Basic Medicine Guizhou Medical University  Guiyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Cheng","sequence":"additional","affiliation":[{"name":"Women's Hospital Zhejiang University School of Medicine  Hangzhou China"},{"name":"Institute of Genetics Zhejiang University School of Medicine  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng\u2010Yun","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Orthopedics Zibo Central Hospital  Zibo China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Mo","sequence":"additional","affiliation":[{"name":"Department of Immunology, National and Guizhou Joint Engineering Laboratory for Cell Engineering and Biomedicine Technique, School of Basic Medicine Guizhou Medical University  Guiyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Orthopedics Zibo Central Hospital  Zibo China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Dong","sequence":"additional","affiliation":[{"name":"Women's Hospital Zhejiang University School of Medicine  Hangzhou China"},{"name":"Institute of Genetics Zhejiang University School of Medicine  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun\u2010Fei","family":"Li","sequence":"additional","affiliation":[{"name":"Women's Hospital Zhejiang University School of Medicine  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Feng","sequence":"additional","affiliation":[{"name":"Institute of 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