{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T19:28:34Z","timestamp":1791660514705,"version":"4.3.4"},"reference-count":36,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2025,12,23]],"date-time":"2025-12-23T00:00:00Z","timestamp":1766448000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,12,23]],"date-time":"2025-12-23T00:00:00Z","timestamp":1766448000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["anatomypubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Developmental Dynamics"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>During ventral body wall closure in amniotes, the skeleton and muscles such as sternum, ribs, and pectoral muscles undergo a striking positional change from the dorso\u2010lateral to mid\u2010ventral region. The precise process of ventral body wall closure has poorly been explored. We have presented and examined two opposing models: the invading closure model, in which musculoskeletal components infiltrate into the mid\u2010ventral tissue, and the replacing closure model, in which the original mid\u2010ventral tissue degenerates to be replaced by the dorso\u2010lateral body wall.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>We developed a new method that enables direct cell labeling with PKH dyes in late chicken embryos. Our PKH labeling has shown that the original mid\u2010ventral tissue, including both mesodermal and ectodermal cells, is progressively confined to the midline as the closure proceeds, contradicting the invading closure model. Moreover, the dorso\u2010lateral tissue, including not only musculoskeletal but also non\u2010musculoskeletal components, eventually contributes to the mid\u2010ventral region. These results support the replacing closure model.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The ventral body wall closure is achieved in a replacing manner. The mid\u2010ventral region of the early body wall is a transient structure to cover the ventral surface, which is eventually eliminated to be replaced by the dorso\u2010lateral region that represents the future definitive body wall.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/dvdy.70106","type":"journal-article","created":{"date-parts":[[2025,12,23]],"date-time":"2025-12-23T09:39:29Z","timestamp":1766482769000},"page":"961-976","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Non\u2010migratory mode of ventral body wall closure: Primary mid\u2010ventral covering is replaced by prospective definitive body wall"],"prefix":"10.1002","volume":"255","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-8856-9424","authenticated-orcid":false,"given":"Kengo","family":"Buma","sequence":"first","affiliation":[{"name":"Department of Zoology Graduate School of Science, Kyoto University  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshiko","family":"Takahashi","sequence":"additional","affiliation":[{"name":"Department of Zoology Graduate School of Science, Kyoto University  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,12,23]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.1939.0002"},{"issue":"4","key":"e_1_2_7_3_1","first-page":"373","article-title":"Studies on the morphogenesis of the mouse sternum: I. 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