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Full genome annotation, the establishment of gene editing techniques, and comprehensive description of reproductive tract morphology and embryogenesis in this species have laid the foundation for functional studies. However, analysis of brown anole oogenesis is still required and vital to optimize genome modification, mutant line establishment, and analyses of the evolution of reproductive developmental mechanisms.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Here, we characterize ovary morphology and gametogenesis in the female brown anole,\n                      <jats:italic>Anolis sagrei,<\/jats:italic>\n                      using brightfield imaging, microCT, histology staining, electron microscopy, and confocal imaging. We define 10 stages of oocyte maturation, which commences inside the oogonial nest within the germinal bed and concludes with the mature follicle ready to ovulate based on follicle size, yolk acquisition, and follicular, cellular, and basement membrane architecture.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>We describe the complete oogenesis of the brown anole in 10 stages and report that oogenesis is highly conserved within iguanians, a suborder of lizards. With our staging framework, we lay the foundation for functional studies of oogenesis and optimized gene\u2010editing.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/dvdy.70112","type":"journal-article","created":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T12:23:20Z","timestamp":1767961400000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Oogenesis and germinal bed morphology of the brown anole (\n                    <i>A<\/i>\n                    .\n                    <i>sagrei<\/i>\n                    )"],"prefix":"10.1002","author":[{"given":"Bonnie K.","family":"Kircher","sequence":"first","affiliation":[{"name":"Department of Genetics University of Texas MD Anderson Cancer Center  Houston Texas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8282-5695","authenticated-orcid":false,"given":"Antonia","family":"Weberling","sequence":"additional","affiliation":[{"name":"All Souls College University of Oxford  Oxford UK"},{"name":"Nuffield Department of Women's and Reproductive Health University of Oxford  Oxford UK"},{"name":"Department of Biochemistry University of Cambridge  Cambridge UK"},{"name":"Stowers Institute for Medical Research  Kansas City Missouri USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erin J.","family":"Vance","sequence":"additional","affiliation":[{"name":"Department of Biosciences Rice University  Houston Texas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Natalia A.","family":"Shylo","sequence":"additional","affiliation":[{"name":"Stowers Institute for Medical Research  Kansas City Missouri USA"},{"name":"Department of Biological and Biomedical Sciences Rowan University  Glassboro New Jersey USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katherine","family":"Starr","sequence":"additional","affiliation":[{"name":"Department of Biology Loyola University Chicago  Chicago Illinois USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zoe B.","family":"Griffin","sequence":"additional","affiliation":[{"name":"Department of Genetics University of Texas MD Anderson Cancer Center  Houston Texas USA"},{"name":"Department of Biosciences Rice University  Houston Texas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hannah","family":"Wilson","sequence":"additional","affiliation":[{"name":"Stowers Institute for Medical Research  Kansas City Missouri USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Melainia","family":"McClain","sequence":"additional","affiliation":[{"name":"Stowers Institute for Medical Research  Kansas City Missouri USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Florian","family":"Hollfelder","sequence":"additional","affiliation":[{"name":"Department of Biochemistry University of Cambridge  Cambridge UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suzannah A.","family":"Williams","sequence":"additional","affiliation":[{"name":"Nuffield Department of Women's and Reproductive Health University of Oxford  Oxford UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas J.","family":"Sanger","sequence":"additional","affiliation":[{"name":"Department of Biology Loyola University Chicago  Chicago Illinois USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard R.","family":"Behringer","sequence":"additional","affiliation":[{"name":"Department of Genetics University of Texas MD Anderson Cancer Center  Houston Texas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2774-3624","authenticated-orcid":false,"given":"Paul A.","family":"Trainor","sequence":"additional","affiliation":[{"name":"Stowers Institute for Medical Research  Kansas City Missouri USA"},{"name":"Department of Cell Biology and Physiology University of Kansas Medical Center  Kansas City Missouri USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,1,9]]},"reference":[{"issue":"961","key":"e_1_2_8_2_1","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1098\/rspb.1962.0019","article-title":"A quantitative and cytological study of oogonia and oocytes in the foetal and neonatal rat","volume":"155","author":"Beaumont HM","year":"1962","journal-title":"Proc R Soc Lond B Biol 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