{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T12:11:26Z","timestamp":1773922286753,"version":"3.50.1"},"reference-count":42,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T00:00:00Z","timestamp":1773878400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T00:00:00Z","timestamp":1773878400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["anatomypubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Developmental Dynamics"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Ambystoma mexicanum<\/jats:italic>\n                      is renowned for its extraordinary regenerative capacity. Regardless of whether progenitor cells arise via dedifferentiation or stem cell activation, these cells must replicate DNA to proliferate before they ultimately differentiate. This rapid cell division increases the likelihood of DNA damage, requiring efficient repair mechanisms.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      We focused on the non\u2010homologous end joining pathway, responsible for repairing double\u2010strand DNA breaks, and investigated the effects of 2 key components\u2014KU70\/80 and LIGIV\u2010 using chemical inhibitors STL127705 and SCR130, respectively. We assessed the impact of these inhibitors on axolotl limb regeneration, finding that treatment with the LIGIV inhibitor (SCR130) led to a positive growth trend in the regenerated area. Histological analysis of re\u2010amputated limbs revealed no major morphological differences between treated and control animals. However, we observed a significant increase in the number of senescent cells in the regenerated tissue after KU70\/80 inhibition (STL127705) at 32\u2009days post\u2010amputation, suggesting that DNA repair inhibition may promote senescence. Furthermore, we detected a dose\u2010dependent increase in DNA damage, indicated by the\n                      <jats:italic>\u03b3<\/jats:italic>\n                      H2AX(Ser139) signal, specifically in cells' nuclei within the regenerated tissues.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>These findings suggest that DNA repair inhibition impacts regeneration, potentially through mechanisms linked to cellular senescence and DNA damage accumulation.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/dvdy.70131","type":"journal-article","created":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T07:51:09Z","timestamp":1773906669000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Role of non\u2010homologous end joining\n                    <scp>DNA<\/scp>\n                    repair during limb regeneration in\n                    <scp>\n                      <i>Ambystoma mexicanum<\/i>\n                    <\/scp>"],"prefix":"10.1002","author":[{"given":"Esteban","family":"Torres\u2010Dimas","sequence":"first","affiliation":[{"name":"Genetics and Molecular Biology Department Centro de Investigaci\u00f3n y Estudios Avanzados del IPN  Mexico city Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4808-9441","authenticated-orcid":false,"given":"Garc\u00eda\u2010Lepe","family":"Ulises Omar","sequence":"additional","affiliation":[{"name":"Genetics and Molecular Biology Department Centro de Investigaci\u00f3n y Estudios Avanzados del IPN  Mexico city Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Espinal\u2010Centeno","family":"Annie","sequence":"additional","affiliation":[{"name":"Molecular and Developmental Complexity Group Unidad de Gen\u00f3mica Avanzada (LANGEBIO), Centro de Investigaci\u00f3n y de Estudios Avanzados del IPN  Guanajuato Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cruz\u2010Ram\u00edrez","family":"Alfredo","sequence":"additional","affiliation":[{"name":"Molecular and Developmental Complexity Group Unidad de Gen\u00f3mica Avanzada (LANGEBIO), Centro de Investigaci\u00f3n y de Estudios Avanzados del IPN  Guanajuato Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0463-9357","authenticated-orcid":false,"given":"Berm\u00fadez\u2010Cruz","family":"Rosa Mar\u00eda","sequence":"additional","affiliation":[{"name":"Genetics and Molecular Biology Department Centro de Investigaci\u00f3n y Estudios Avanzados del IPN  Mexico city 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