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During injury response, fibroblasts and endothelial cells accumulate at the site of damage, and cardiomyocyte cell cycle reentry allows cardiac muscle regrowth. It is relevant to understand how the different cell types communicate with each other to coordinate regeneration.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      We present an\n                      <jats:italic>in silico<\/jats:italic>\n                      meta\u2010analysis of ligand\u2013receptor (LR) interactions among\n                      <jats:italic>periostin b<\/jats:italic>\n                      <jats:sup>+<\/jats:sup>\n                      fibroblasts,\n                      <jats:italic>kdlr<\/jats:italic>\n                      <jats:sup>+<\/jats:sup>\n                      endothelial cells,\n                      <jats:italic>sox10<\/jats:italic>\n                      \u2010derived and rest of ventricular cardiomyocytes, at 7\u2009days post\u2010injury. Using bulk RNA\u2010seq data sets from fluorophore\u2010activated cell\u2010sorted populations, we selected for differentially expressed genes encoding LR pairs. Human\u2010centric interaction data from the OmniPath database were adapted to zebrafish data through ortholog mapping to reconstruct a comprehensive interactome. We observed that fibroblasts and, to a lesser extent, endothelial cells emerged as signaling hubs, while cardiomyocytes primarily acted as signal recipients. Network analysis, combining PageRank, expression change, and literature\u2010based novelty, revealed both known and novel candidate genes in regeneration, and allowed pathway enrichment analysis. An interactive web tool enables exploration of the ranked interaction data set, providing a systematic resource to guide future functional studies.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>This study provides a systematic and unbiased map of regenerative signaling in the zebrafish heart, establishing a resource to guide functional investigations.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/dvdy.70158","type":"journal-article","created":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T12:01:57Z","timestamp":1781006517000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["In silico analysis of putative interactions identifies fibroblasts as hubs for cell\u2013cell communication during early regeneration of the zebrafish heart"],"prefix":"10.1002","author":[{"given":"Jo\u00e3o A. 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