{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T13:00:39Z","timestamp":1781010039342,"version":"3.54.1"},"reference-count":25,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T00:00:00Z","timestamp":1780963200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T00:00:00Z","timestamp":1780963200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004281","name":"Narodowe Centrum Nauki","doi-asserted-by":"publisher","award":["2022\/47\/B\/NZ3\/02697"],"award-info":[{"award-number":["2022\/47\/B\/NZ3\/02697"]}],"id":[{"id":"10.13039\/501100004281","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004281","name":"Narodowe Centrum Nauki","doi-asserted-by":"publisher","award":["2017\/27\/B\/NZ9\/02086"],"award-info":[{"award-number":["2017\/27\/B\/NZ9\/02086"]}],"id":[{"id":"10.13039\/501100004281","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["anatomypubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Developmental Dynamics"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Advances in reproductive and cellular biotechnology have significantly enhanced the ability to study, conserve, and manipulate species within the\n                      <jats:italic>Bovinae<\/jats:italic>\n                      family, including domestic cattle and the endangered European bison (wisent). Central to the success of such technologies, including in vitro fertilization, somatic cell nuclear transfer, and the culture of pluripotent stem cells (PSCs), is the chromosome stability of cultured cells and embryos.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Here, we present the design and application of a specific centromere Cot\u20101 DNA probe for fluorescence in situ hybridization (FISH). We demonstrate its utility in assessing chromosomal stability of bovine and wisent somatic cells and in vitro\u2010produced embryos. The probe shows high specificity for\n                      <jats:italic>Bovinae<\/jats:italic>\n                      cells and can be used on both metaphase plates and interphase nuclei, enabling rapid cytogenetic analysis even in slow\u2010dividing or senescent cells.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      The newly generated Cot\u20101 DNA\u2010based FISH probe provides a rapid and reliable method for species\u2010specific cytogenetic monitoring in\n                      <jats:italic>Bovinae<\/jats:italic>\n                      . Furthermore, it allows accurate monitoring of chromosomal stability and quality control in PSC systems reliant on feeder layers and offers a streamlined approach for monitoring embryo chromosomal integrity in vitro. Broader application of this method may improve outcomes in reproductive technologies, conservation programs, and the generation of stable cell lines for biotechnological and agricultural use.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/dvdy.70159","type":"journal-article","created":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T12:04:22Z","timestamp":1781006662000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Application of centromeric fluorescence in situ hybridization probes for embryological and cell studies\u2014A practical approach to validate chromosome stability in\n                    <i>Bovinae<\/i>"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9649-2422","authenticated-orcid":false,"given":"Zofia E.","family":"Madeja","sequence":"first","affiliation":[{"name":"Department of Genetics and Animal Breeding, Faculty of Veterinary Medicine and Animal Sciences Poznan University of Life Sciences  Poznan Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4238-0414","authenticated-orcid":false,"given":"Izabela","family":"Szczerbal","sequence":"additional","affiliation":[{"name":"Department of Genetics and Animal Breeding, Faculty of Veterinary Medicine and Animal Sciences Poznan University of Life Sciences  Poznan Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-8546-7175","authenticated-orcid":false,"given":"Marcelina H.","family":"Osyra","sequence":"additional","affiliation":[{"name":"Department of Genetics and Animal Breeding, Faculty of Veterinary Medicine and Animal Sciences Poznan University of Life Sciences  Poznan Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7165-1386","authenticated-orcid":false,"given":"Marta M.","family":"Zi\u0119tek","sequence":"additional","affiliation":[{"name":"Department of Experimental Embryology Institute of Genetics and Animal Biotechnology, Polish Academy of Sciences  Magdalenka Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1232-4286","authenticated-orcid":false,"given":"Agnieszka","family":"Bernat","sequence":"additional","affiliation":[{"name":"Department of Experimental Embryology Institute of Genetics and Animal Biotechnology, Polish Academy of Sciences  Magdalenka Poland"},{"name":"Laboratory of Photobiology and Molecular Diagnostics, Intercollegiate Faculty of Biotechnology University of Gda\u0144sk &amp; Medical University of Gda\u0144sk  Gda\u0144sk Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,9]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dex286"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.15252\/embj.201593103"},{"issue":"12","key":"e_1_2_9_4_1","first-page":"3107","article-title":"Persistent oxidative stress in chromosomally unstable cells","volume":"63","author":"Limoli CL","year":"2003","journal-title":"Cancer Res"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/cdd.2011.9"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13039\u2010015\u20100133\u20108"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stemcr.2016.01.015"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.282.5391.1145"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm3190"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2008.12.007"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.104.029413"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1716161115"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.animal.2023.100803"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt922"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani13050944"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010020\u201079384\u2010x"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12015\u2010010\u20109224\u20104"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms26094327"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010025\u201065778\u2010w"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/gbe\/evz061"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/cpr.13189"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41580\u2010020\u201000292\u2010z"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fufo.2025.100555"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/asj.13272"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.3168\/jdsc.2022\u20100275"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells12050790"}],"container-title":["Developmental Dynamics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dvdy.70159","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/dvdy.70159","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dvdy.70159","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T12:04:23Z","timestamp":1781006663000},"score":1,"resource":{"primary":{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/10.1002\/dvdy.70159"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,9]]},"references-count":25,"alternative-id":["10.1002\/dvdy.70159"],"URL":"https:\/\/doi.org\/10.1002\/dvdy.70159","archive":["Portico"],"relation":{},"ISSN":["1058-8388","1097-0177"],"issn-type":[{"value":"1058-8388","type":"print"},{"value":"1097-0177","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,9]]},"assertion":[{"value":"2026-01-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-27","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-09","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"dvdy.70159"}}