{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T05:15:30Z","timestamp":1783487730434,"version":"3.55.0"},"reference-count":78,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T00:00:00Z","timestamp":1783382400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T00:00:00Z","timestamp":1783382400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["anatomypubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Developmental Dynamics"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      One of the most characteristic morphogenetic processes in\n                      <jats:italic>Drosophila<\/jats:italic>\n                      is the 360\u00b0 rotation of the male pupal genital disc. This movement is driven by the myosin Myo1D, whose expression in the genital disc is controlled by the Hox gene\n                      <jats:italic>Abdominal\u2010B<\/jats:italic>\n                      (\n                      <jats:italic>Abd\u2010B<\/jats:italic>\n                      ). The rotation takes place in contact and relative to the posterior abdomen, yet the contribution of abdominal tissues has remained unclear.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Here we show that normal genital disc circumrotation requires active remodeling of posterior abdominal larval epidermal cells that contact the rotating terminalia. Preventing apoptosis in these cells, or increasing EGFR signaling, delays their extrusion and results in incomplete rotation without altering rotational chirality. In parallel, elimination of extracellular matrix (ECM) by Metalloproteinase 1 in these cells, although without preventing their extrusion, is also strictly required for genital disc circumrotation. Inhibition of this metalloproteinase activity leads to persistence of Collagen IV and incomplete rotation, revealing an independent requirement for ECM clearance at the disc\u2013abdomen interface. By contrast, genetic conditions that prevent formation or elimination of the male A7 segment do not necessarily impair genital disc rotation, demonstrating that A7 suppression and circumrotation are separable processes.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>These findings identify posterior abdominal tissue remodeling as an essential extrinsic requirement that enables genital disc circumrotation.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/dvdy.70170","type":"journal-article","created":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T04:28:16Z","timestamp":1783484896000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<i>Drosophila<\/i>\n                    male genitalia rotation depends on permissive remodeling of the posterior abdomen"],"prefix":"10.1002","author":[{"given":"Nuria","family":"Prieto","sequence":"first","affiliation":[{"name":"Centro de Biolog\u00eda Molecular Severo Ochoa (CBM) CSIC\u2010UAM, Universidad Aut\u00f3noma de Madrid  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Foronda","sequence":"additional","affiliation":[{"name":"Department of Biology Universidad Aut\u00f3noma de Madrid  Madrid Spain"},{"name":"Instituto Universitario de Biolog\u00eda Molecular\u2010IUBM Universidad Aut\u00f3noma de Madrid  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paloma","family":"Mart\u00edn","sequence":"additional","affiliation":[{"name":"Centro de Biolog\u00eda Molecular Severo Ochoa (CBM) CSIC\u2010UAM, Universidad Aut\u00f3noma de Madrid  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"El\u00e9anor","family":"Simon","sequence":"additional","affiliation":[{"name":"Centro de Biolog\u00eda Molecular Severo Ochoa (CBM) CSIC\u2010UAM, Universidad Aut\u00f3noma de Madrid  Madrid Spain"},{"name":"INRAE Universit\u00e9 C\u00f4te d'Azur, Sophia Agrobiotech Institute (ISA)  Sophia Antipolis France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcus","family":"Bischoff","sequence":"additional","affiliation":[{"name":"Biomedical Sciences Research Complex University of St Andrews  St Andrews UK"},{"name":"Centre of Biophotonics University of St Andrews  St Andrews UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"St\u00e9phane","family":"Noselli","sequence":"additional","affiliation":[{"name":"Institut de Biologie Valrose iBV Universit\u00e9 C\u00f4te d'Azur, Inserm, CNRS  Nice France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5688-2303","authenticated-orcid":false,"given":"Ernesto","family":"S\u00e1nchez\u2010Herrero","sequence":"additional","affiliation":[{"name":"Centro de Biolog\u00eda Molecular Severo Ochoa (CBM) CSIC\u2010UAM, Universidad Aut\u00f3noma de Madrid  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,7]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.15252\/embr.201438972"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00848062"},{"key":"e_1_2_9_4_1","first-page":"1","article-title":"Anatomie und Variabilit\u00e4t des Geschlechtsapparates von Drosophila melanogaster (Meigen)","volume":"148","author":"Gleichauf R","year":"1936","journal-title":"Z Wiss Zool"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature04625"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.058958"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature04623"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2010.08.056"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms10074"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.00460"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2012.11.013"},{"key":"e_1_2_9_12_1","first-page":"1","article-title":"Morphogenesis of the epidermis of adult abdomen of Drosophila","volume":"60","author":"Madhavan MM","year":"1980","journal-title":"J Embryol Exp Morphol"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.02728"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1002874"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1108431108"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.23791"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1006622"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460\u20102075.1990.tb07876.x"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460\u20102075.1990.tb07439.x"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092\u20108674(85)90014\u20105"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.111.2.437"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00848355"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0092\u20108674(00)81819\u2010x"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0092\u20108674(00)80528\u20100"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.117.1.219"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.179606"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.01046\u201010"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.170811"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.035410"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1387\/ijdb.103109rk"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.05338"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41420\u2010025\u201002916\u20109"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0034063"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.10936"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep09015"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.00205"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.037861"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1004717"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2010.01.060"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.120.8.2121"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.139865"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2011.12.033"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0092\u20108674(00)81765\u20101"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.3002547"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0092\u20108674(00)81764\u2010x"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.3002823"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.124.19.3871"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2004.09.001"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.125.1.1"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mod.2015.07.016"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7601401"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2022.01.045"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.261408198"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1038\/313108a0"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb3420"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/iyac030"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.152652"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.24958"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.06565"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2022.105609"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469\u2010185X.2007.00029.x"},{"key":"e_1_2_9_62_1","volume-title":"Morphology and Evolution of the Insect Abdomen: With Special Reference to Developmental Patterns and Their Bearings Upon Systematics","author":"Matsuda R","year":"2013"},{"key":"e_1_2_9_63_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10709\u2010008\u20109339\u20106"},{"key":"e_1_2_9_64_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010018\u201036301\u20107"},{"key":"e_1_2_9_65_1","doi-asserted-by":"publisher","DOI":"10.4161\/fly.22109"},{"key":"e_1_2_9_66_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092\u20108674(86)90627\u20106"},{"key":"e_1_2_9_67_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1004989"},{"key":"e_1_2_9_68_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.133"},{"key":"e_1_2_9_69_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.124.4.761"},{"key":"e_1_2_9_70_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.214202"},{"key":"e_1_2_9_71_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.274.5285.252"},{"key":"e_1_2_9_72_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn.2656"},{"key":"e_1_2_9_73_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0092\u20108674(00)81818\u20108"},{"key":"e_1_2_9_74_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1089035"},{"key":"e_1_2_9_75_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.24045"},{"key":"e_1_2_9_76_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1005957107"},{"key":"e_1_2_9_77_1","volume-title":"Drosophila Protocols","author":"Sullivan W","year":"2000"},{"key":"e_1_2_9_78_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-2772-2_2"},{"key":"e_1_2_9_79_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2019"}],"container-title":["Developmental Dynamics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dvdy.70170","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/dvdy.70170","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dvdy.70170","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T04:28:18Z","timestamp":1783484898000},"score":1,"resource":{"primary":{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/10.1002\/dvdy.70170"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,7]]},"references-count":78,"alternative-id":["10.1002\/dvdy.70170"],"URL":"https:\/\/doi.org\/10.1002\/dvdy.70170","archive":["Portico"],"relation":{},"ISSN":["1058-8388","1097-0177"],"issn-type":[{"value":"1058-8388","type":"print"},{"value":"1097-0177","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,7]]},"assertion":[{"value":"2026-03-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-26","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-07","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"dvdy.70170"}}