{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T13:58:26Z","timestamp":1791295106581,"version":"4.1.0"},"reference-count":37,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T00:00:00Z","timestamp":1791244800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T00:00:00Z","timestamp":1791244800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["anatomypubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Developmental Dynamics"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Pharyngeal pouch formation is fundamental to vertebrate craniofacial development, but molecular mechanisms underlying posterior pouch formation remain incompletely understood. Retinoic acid (RA) signaling is required for posterior pharyngeal development, and Hox genes are established RA targets. However, their genetic requirement in posterior pouch formation has not been established.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Zebrafish lacking both\n                      <jats:italic>hoxba<\/jats:italic>\n                      and\n                      <jats:italic>hoxbb<\/jats:italic>\n                      clusters\u2014teleost\u2010specific duplicates of the ancestral HoxB cluster\u2014exhibited severe loss of posterior pharyngeal pouches and associated pharyngeal skeletal elements, accompanied by reduced expression of the pouch marker\n                      <jats:italic>pax1a<\/jats:italic>\n                      . Alcian blue analysis revealed highly similar losses of posterior pharyngeal skeletal elements in\n                      <jats:italic>hoxba;hoxbb<\/jats:italic>\n                      and RA\u2010deficient\n                      <jats:italic>aldh1a2<\/jats:italic>\n                      mutants. Pharyngeal expression of\n                      <jats:italic>hoxb1a<\/jats:italic>\n                      and\n                      <jats:italic>hoxb1b<\/jats:italic>\n                      was reduced in\n                      <jats:italic>aldh1a2<\/jats:italic>\n                      mutants. Combined knockdown of\n                      <jats:italic>hoxb1a<\/jats:italic>\n                      and\n                      <jats:italic>hoxb1b<\/jats:italic>\n                      caused variable, incompletely penetrant posterior pouch abnormalities, supporting their contribution to HoxB\u2010cluster function.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      These results provide genetic evidence that the\n                      <jats:italic>hoxba<\/jats:italic>\n                      and\n                      <jats:italic>hoxbb<\/jats:italic>\n                      clusters are redundantly required for posterior pharyngeal pouch development. The highly similar posterior pharyngeal abnormalities in\n                      <jats:italic>hoxba;hoxbb<\/jats:italic>\n                      and\n                      <jats:italic>aldh1a2<\/jats:italic>\n                      mutants, together with reduced\n                      <jats:italic>hoxb1a<\/jats:italic>\n                      and\n                      <jats:italic>hoxb1b<\/jats:italic>\n                      expression, support a functional association between RA signaling and HoxB\u2010cluster activity. Together, these findings identify the duplicated HoxB\u2010derived clusters as important genetic components of RA\u2010associated posterior pharyngeal development.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/dvdy.70195","type":"journal-article","created":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T13:17:17Z","timestamp":1791292637000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Zebrafish\n                    <i>hoxba<\/i>\n                    and\n                    <i>hoxbb<\/i>\n                    clusters are redundantly required for posterior pharyngeal pouch development"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-8684-8345","authenticated-orcid":false,"given":"Sohju","family":"Toyama","sequence":"first","affiliation":[{"name":"Division of Life Science Graduate School of Science and Engineering, Saitama University  Saitama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-8321-9919","authenticated-orcid":false,"given":"Nanami","family":"Iwamatsu","sequence":"additional","affiliation":[{"name":"Division of Life Science Graduate School of Science and Engineering, Saitama University  Saitama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-6567-8775","authenticated-orcid":false,"given":"Junpei","family":"Imagawa","sequence":"additional","affiliation":[{"name":"Division of Life Science Graduate School of Science and Engineering, Saitama University  Saitama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-4337-4544","authenticated-orcid":false,"given":"Takumi","family":"Sugawara","sequence":"additional","affiliation":[{"name":"Division of Life Science Graduate School of Science and Engineering, Saitama University  Saitama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-3414-4122","authenticated-orcid":false,"given":"Renka","family":"Fujii","sequence":"additional","affiliation":[{"name":"Division of Life Science Graduate School of Science and Engineering, Saitama University  Saitama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2591-009X","authenticated-orcid":false,"given":"Morimichi","family":"Kikuchi","sequence":"additional","affiliation":[{"name":"Division of Life Science Graduate School of Science and Engineering, Saitama University  Saitama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-9149-1605","authenticated-orcid":false,"given":"Yuki","family":"Kawabe","sequence":"additional","affiliation":[{"name":"Division of Life Science Graduate School of Science and Engineering, Saitama University  Saitama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-7298-6513","authenticated-orcid":false,"given":"Yuzuki","family":"Tanaka","sequence":"additional","affiliation":[{"name":"Division of Life Science Graduate School of Science and Engineering, Saitama University  Saitama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5618-7113","authenticated-orcid":false,"given":"Akinori","family":"Kawamura","sequence":"additional","affiliation":[{"name":"Division of Life Science Graduate School of Science and Engineering, Saitama University  Saitama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,6]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.220.4594.268"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/1521\u20101878(200101)23:1<54::AID\u2010BIES1007>3.0.CO;2\u20105"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0021\u20108782.2004.00345.x"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.2000.9842"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.128.16.3081"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.20905"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.00463"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.10137"},{"issue":"9","key":"e_1_2_8_10_1","doi-asserted-by":"crossref","first-page":"969","DOI":"10.1096\/fasebj.10.9.8801179","article-title":"Retinoids and Hox genes","volume":"10","author":"Marshall H","year":"1996","journal-title":"FASEB J"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.282.5394.1711"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.127.8.1553"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/355516a0"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.122.10.3217"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.2000.9683"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.126.22.5027"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.198325"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.129.12.2851"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2010.10.011"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.129.10.2339"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471\u2010213X\u201014\u201025"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.105889.3"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586\u2010019\u20101064\u2010z"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586\u2010019\u20101057\u2010y"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.121.7.1989"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.9.13.1667"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1085672"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.993302"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2403809121"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/iyaf218"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.10057"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/370567a0"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.2002.0701"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mod.2020.103598"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.130039"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.194738"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1002030302"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1007\/978\u20101\u20104939\u20101459\u20103_5"}],"container-title":["Developmental Dynamics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dvdy.70195","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/dvdy.70195","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dvdy.70195","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T13:17:18Z","timestamp":1791292638000},"score":1,"resource":{"primary":{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/10.1002\/dvdy.70195"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,6]]},"references-count":37,"alternative-id":["10.1002\/dvdy.70195"],"URL":"https:\/\/doi.org\/10.1002\/dvdy.70195","archive":["Portico"],"relation":{},"ISSN":["1058-8388","1097-0177"],"issn-type":[{"value":"1058-8388","type":"print"},{"value":"1097-0177","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,6]]},"assertion":[{"value":"2026-04-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-28","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-06","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"dvdy.70195"}}