{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T23:15:16Z","timestamp":1778800516259,"version":"3.51.4"},"reference-count":51,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2024,6,1]],"date-time":"2024-06-01T00:00:00Z","timestamp":1717200000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100020595","name":"National Science and Technology Council","doi-asserted-by":"publisher","award":["NSTC 111\u20102320\u2010B\u2010030 \u2010007\u2010MY3"],"award-info":[{"award-number":["NSTC 111\u20102320\u2010B\u2010030 \u2010007\u2010MY3"]}],"id":[{"id":"10.13039\/100020595","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002811","name":"Cathay General Hospital","doi-asserted-by":"publisher","award":["112\u2010CGH\u2010FJU\u201003"],"award-info":[{"award-number":["112\u2010CGH\u2010FJU\u201003"]}],"id":[{"id":"10.13039\/501100002811","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009974","name":"Cardinal Tien Hospital","doi-asserted-by":"publisher","award":["CTH\u2010112A\u20102209"],"award-info":[{"award-number":["CTH\u2010112A\u20102209"]}],"id":[{"id":"10.13039\/501100009974","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["anatomypubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Developmental Dynamics"],"published-print":{"date-parts":[[2024,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Approximately 7% of the males exhibit reduced fertility; however, the regulatory genes and pathways involved remain largely unknown. TBC1 domain family member 21 (TBC1D21) contains a conserved RabGAP catalytic domain that induces GDP\/GTP exchange to inactivate Rabs by interacting with microtubules. We previously reported that <jats:italic>Tbc1d21\u2010null<\/jats:italic> mice exhibit severe sperm tail defects with a disrupted axoneme, and that TBC1D21 interacts with RAB10. However, the pathological mechanisms underlying the <jats:italic>Tbc1d21<\/jats:italic> loss<jats:italic>\u2010<\/jats:italic>induced sperm tail defects remain unknown.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Murine sperm from wild\u2010type and <jats:italic>Tbc1d21<\/jats:italic>\u2010null mice were comparatively analyzed using proteomic assays. Over 1600 proteins were identified, of which 15 were significantly up\u2010regulated in <jats:italic>Tbc1d21<\/jats:italic>\u2010null sperm. Notably, several tektin (TEKT) family proteins, belonging to a type of intermediate filament critical for stabilizing the microtubular structure of cilia and flagella, were significantly up\u2010regulated in <jats:italic>Tbc1d21<\/jats:italic><jats:sup><jats:italic>\u2212\/\u2212<\/jats:italic><\/jats:sup> sperm. We also found that TBC1D21 interacts with TEKT1. In addition, TEKT1 co\u2010localized with RAB10 during sperm tail formation. Finally, we found <jats:italic>Tbc1d21<\/jats:italic>\u2010null sperm exhibited abnormal accumulation of TEKT1 in the midpiece region, accompanied by disrupted axonemal structures.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These results reveal that TBC1D21 modulates TEKTs protein localization in the axonemal transport system during sperm tail formation.<\/jats:p><\/jats:sec>","DOI":"10.1002\/dvdy.716","type":"journal-article","created":{"date-parts":[[2024,6,1]],"date-time":"2024-06-01T05:34:55Z","timestamp":1717220095000},"page":"1024-1035","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Proteomic profiling of <i>TBC1 domain family member 21<\/i>\u2010null sperms reveals the critical roles of <scp>TEKT<\/scp> 1 in their tail defects"],"prefix":"10.1002","volume":"253","author":[{"given":"Pei\u2010Yi","family":"Pan","sequence":"first","affiliation":[{"name":"Graduate Institute of Biomedical and Pharmaceutical Science Fu\u2010Jen Catholic University  New Taipei City Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chih\u2010Chun","family":"Ke","sequence":"additional","affiliation":[{"name":"Department of Urology En Chu Kong Hospital  New Taipei City Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ya\u2010Yun","family":"Wang","sequence":"additional","affiliation":[{"name":"Graduate Institute of Biomedical and Pharmaceutical Science Fu\u2010Jen Catholic University  New Taipei City Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu\u2010Hua","family":"Lin","sequence":"additional","affiliation":[{"name":"Division of Urology, Department of Surgery Cardinal Tien Hospital  New Taipei City Taiwan"},{"name":"Department of Chemistry Fu Jen Catholic University  New Taipei City Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei\u2010Chi","family":"Ku","sequence":"additional","affiliation":[{"name":"School of Medicine Fu Jen Catholic University  New Taipei City Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chin\u2010Fong","family":"Au","sequence":"additional","affiliation":[{"name":"Division of Urology, Department of Surgery Far Eastern Memorial Hospital  New Taipei City Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chying\u2010Chyuan","family":"Chan","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Taipei City Hospital, Zhongxing Branch and Branch for Women and Children  Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chia\u2010Yen","family":"Huang","sequence":"additional","affiliation":[{"name":"Gynecologic Cancer Center, Department of Obstetrics and Gynecology Cathay General Hospital  Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1574-1579","authenticated-orcid":false,"given":"Ying\u2010Hung","family":"Lin","sequence":"additional","affiliation":[{"name":"Graduate Institute of Biomedical and Pharmaceutical Science Fu\u2010Jen Catholic University  New Taipei City Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,6]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0015\u20100282(02)03105\u20109"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.12968\/bjon.2016.25.18.S35"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140\u20106736(20)32667\u20102"},{"key":"e_1_2_9_5_1","volume-title":"Clinical Management of Male Infertility","author":"Barak S","year":"2000"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41585\u2010018\u20100051\u20108"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg911"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1530\/REP\u201015\u20100310"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/biolre\/iox096"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1530\/REP\u201018\u20100457"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms14058912"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceb.2010.04.007"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1080\/21541248.2017.1353847"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1999.9302"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod62.4.1084"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1121173109"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2002\u2010220893"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1002969"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms18010097"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature04847"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2006.04.039"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102605.2010.01126.x"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1009020"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2018355118"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/biolre\/ioac069"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb\u20102008\u20109\u20107\u2010229"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1078\/0171\u20109335\u201000097"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.2108\/zsj.31.101"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod66.1.241"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.24.18.7958\u20107964.2004"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.2108\/zsj.27.755"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dem400"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.20025"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.20957"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.21352"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.20331"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.20486"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.06\u20107035com"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M500322\u2010MCP200"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.20804"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.2164\/jandrol.109.009456"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.00154\u201019"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M114.568949"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.18-21-08912.1998"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra040319"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00418\u2010013\u20101101\u20101"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainresbull.2009.06.004"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.4161\/rdis.25378"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddw081"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2009.21"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2007.261"},{"issue":"3","key":"e_1_2_9_52_1","first-page":"219","article-title":"Dual roles of 17\u2010beta estradiol in estrogen receptor\u2010dependent growth inhibition in renal cell carcinoma","volume":"13","author":"Chen KC","year":"2016","journal-title":"Cancer Genomics Proteomics"}],"container-title":["Developmental Dynamics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dvdy.716","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T18:02:55Z","timestamp":1760378575000},"score":1,"resource":{"primary":{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/10.1002\/dvdy.716"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,6]]},"references-count":51,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2024,11]]}},"alternative-id":["10.1002\/dvdy.716"],"URL":"https:\/\/doi.org\/10.1002\/dvdy.716","archive":["Portico"],"relation":{},"ISSN":["1058-8388","1097-0177"],"issn-type":[{"value":"1058-8388","type":"print"},{"value":"1097-0177","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,6]]},"assertion":[{"value":"2023-12-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-04-22","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-06-01","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}