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This study establishes a hydrocortisone injection procedure (incl. dosage) that consistently inhibits all conjunctival papillae in the embryonic chicken eye. The effects of this hydrocortisone treatment on apoptosis, vasculature, and placode\u2010related gene expression were assessed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Hydrocortisone treatment does not increase apoptotic cell death or have a major effect on the ciliary artery or vascular plexus in the eye. <jats:italic>\u03b2\u2010catenin<\/jats:italic> and <jats:italic>Eda<\/jats:italic> expression levels were not significantly altered following hydrocortisone treatment, despite the absence of conjunctival papillae. Notably, <jats:italic>Fgf20<\/jats:italic> expression was significantly reduced following hydrocortisone treatment, and the distribution of <jats:italic>\u03b2\u2010catenin<\/jats:italic> was altered.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our study showed that conjunctival papillae induction begins as early as HH27.5 (E5.5). Hydrocortisone treatment reduces <jats:italic>Fgf20<\/jats:italic> expression independently of <jats:italic>\u03b2\u2010catenin<\/jats:italic> and <jats:italic>Eda<\/jats:italic> and may instead affect other members of the <jats:italic>Wnt<\/jats:italic>\/<jats:italic>\u03b2\u2010catenin<\/jats:italic> or <jats:italic>Eda\/Edar<\/jats:italic> pathways, or it may affect the ability of morphogens to diffuse through the extracellular matrix. This study contributes to a growing profile of gene expression data during placode development and enhances our understanding of how some vertebrate eyes develop these fascinating bones.<\/jats:p><\/jats:sec>","DOI":"10.1002\/dvdy.729","type":"journal-article","created":{"date-parts":[[2024,8,3]],"date-time":"2024-08-03T07:20:14Z","timestamp":1722669614000},"page":"74-93","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Hydrocortisone treatment as a tool to study conjunctival placode induction"],"prefix":"10.1002","volume":"254","author":[{"given":"Paige M.","family":"Drake","sequence":"first","affiliation":[{"name":"Department of Medical Neuroscience Dalhousie University  Halifax Nova Scotia Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4883-2677","authenticated-orcid":false,"given":"Tamara 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