{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T23:07:57Z","timestamp":1785798477518,"version":"3.56.0"},"reference-count":41,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2024,9,25]],"date-time":"2024-09-25T00:00:00Z","timestamp":1727222400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["19H03858"],"award-info":[{"award-number":["19H03858"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["23K27803"],"award-info":[{"award-number":["23K27803"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["anatomypubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Developmental Dynamics"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Embryonic craniofacial development involves several cellular and molecular events that are evolutionarily conserved among vertebrates. Vertebrate models such as mice and zebrafish have been used to investigate the molecular and cellular etiologies underlying human craniofacial disorders, including orofacial clefts. However, the molecular mechanisms underlying embryonic development in these two species are unknown. Therefore, elucidating the shared mechanisms of craniofacial development between disease models is crucial to understanding the underlying mechanisms of phenotypes in individual species.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We selected mice and zebrafish as model organisms to compare various events during embryonic craniofacial development. We identified genes (<jats:italic>Sox9<\/jats:italic>, <jats:italic>Zfhx3<\/jats:italic> and <jats:italic>4<\/jats:italic>, <jats:italic>Cjun<\/jats:italic>, and <jats:italic>Six1<\/jats:italic>) exhibiting similar temporal expression patterns between these species through comprehensive and stage\u2010matched gene expression analyses. Expression analysis revealed similar gene expression in hypothetically corresponding tissues, such as the mice palate and zebrafish ethmoid plate. Furthermore, loss\u2010of\u2010function analysis of <jats:italic>Zfhx4\/zfhx4<\/jats:italic>, a causative gene of human craniofacial anomalies including orofacial cleft, in both species resulted in deformed skeletal elements such as the palatine and ethmoid plate in mice and zebrafish, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These results demonstrate that these disease models share common molecular mechanisms, highlighting their usefulness in modeling craniofacial defects in humans.<\/jats:p><\/jats:sec>","DOI":"10.1002\/dvdy.740","type":"journal-article","created":{"date-parts":[[2024,9,25]],"date-time":"2024-09-25T11:35:42Z","timestamp":1727264142000},"page":"257-271","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Expression analysis of genes including <i>Zfhx4<\/i> in mice and zebrafish reveals a temporospatial conserved molecular basis underlying craniofacial development"],"prefix":"10.1002","volume":"254","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-6633-6806","authenticated-orcid":false,"given":"Shujie","family":"Liu","sequence":"first","affiliation":[{"name":"R&amp;D, Safety Science Research, Kao Corporation  Kawasaki Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Orthodontics and Dentofacial Orthopedics Osaka University Graduate School of Dentistry  Suita Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3953-9033","authenticated-orcid":false,"given":"Makoto","family":"Kashima","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biological Science College of Science and Engineering, Aoyama Gakuin University  Sagamihara Japan"},{"name":"Department of Biomolecular Science, Faculty of Science Toho University  Funabashi 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