{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T10:54:33Z","timestamp":1791975273291,"version":"4.4.0"},"reference-count":30,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,10,10]],"date-time":"2024-10-10T00:00:00Z","timestamp":1728518400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["21580367"],"award-info":[{"award-number":["21580367"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["anatomypubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Developmental Dynamics"],"published-print":{"date-parts":[[2025,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Eph receptors and ephrin ligands, the transmembrane proteins, function as a mechanism of communication between cells. Therefore, we intended to explore the expression array of EphB2 and EphB4 receptors and ephrin\u2010B1 ligand in postnatal developing mouse epididymis during 1\u2009day to 8\u2009weeks using RT\u2010PCR amplification and immunofluorescence staining.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>RT\u2010PCR analysis indicated that the expression levels of EphB2, EphB4, and ephrin\u2010B1 in the epididymis declined with the advancement of age during the initial phases of postnatal development and stayed relatively near to adult levels until 4\u2009weeks. We discovered that the predominant compartments expressing EphB2\/B4 and ephrin\u2010B1 emerged in the excurrent duct epithelia of postnatal developing epididymis until 3\u2009weeks. Consequently, even before spermatozoa reach the excurrent duct in epididymis, at the age of 3\u2009weeks, the epididymal excurrent duct system exhibits characteristics similar to those of an adult in terms of expression of EphB2\/B4 and ephrin\u2010B1. Moreover, ephrin\u2010B1 was expressed in epididymal epithelial cells throughout the development and EphB4 was expressed only in early postnatal stages while basal cells expressed EphB4 throughout the postnatal development.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The study represents the first expression analysis of ephrin\u2010B1, EphB2, and EphB4 in the normal mouse epididymis during the postnatal development.<\/jats:p><\/jats:sec>","DOI":"10.1002\/dvdy.752","type":"journal-article","created":{"date-parts":[[2024,10,11]],"date-time":"2024-10-11T00:40:52Z","timestamp":1728607252000},"page":"478-493","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["<scp>EphB2<\/scp>, <scp>EphB4<\/scp>, and ephrin\u2010<scp>B1<\/scp> expression and localization in postnatal developing epididymis in mice"],"prefix":"10.1002","volume":"254","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8778-2417","authenticated-orcid":false,"given":"Md. Royhan","family":"Gofur","sequence":"first","affiliation":[{"name":"Department of Veterinary and Animal Sciences University of Rajshahi  Rajshahi Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1479-8706","authenticated-orcid":false,"given":"Kazushige","family":"Ogawa","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Anatomy, Graduate School of Veterinary Science Osaka Metropolitan University  Izumisano, Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,10,10]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2006.09.010"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.4103\/1008\u2010682X.155540"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441\u2010012\u20101381\u20100"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0038457"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2005.11.043"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2014\u20101341"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-012515400-0\/50027-0"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2605.1985.tb00858.x"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.5455\/javar.2024.k801"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1001780103"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1530\/REP\u201015\u20100533"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1530\/rep.0.1250871"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/dmn055"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2008.03.011"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm1662"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm856"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v21.i2.453"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.4161\/19336918.2014.970012"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00418-013-1129-2"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00418-011-0867-2"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2008.07.019"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/andr.12523"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.repbio.2020.06.001"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a008227"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng.2007.11"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00418-011-0849-4"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diff.2010.06.003"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1530\/REP\u201011\u20100245"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E10\u201003\u20100242"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijo_00000364"}],"container-title":["Developmental Dynamics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dvdy.752","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,14]],"date-time":"2025-10-14T00:18:20Z","timestamp":1760401100000},"score":1,"resource":{"primary":{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/10.1002\/dvdy.752"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,10,10]]},"references-count":30,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2025,6]]}},"alternative-id":["10.1002\/dvdy.752"],"URL":"https:\/\/doi.org\/10.1002\/dvdy.752","archive":["Portico"],"relation":{},"ISSN":["1058-8388","1097-0177"],"issn-type":[{"value":"1058-8388","type":"print"},{"value":"1097-0177","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,10,10]]},"assertion":[{"value":"2024-07-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-09-02","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-10-10","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}