{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T05:45:03Z","timestamp":1773121503197,"version":"3.50.1"},"reference-count":41,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2019,7,17]],"date-time":"2019-07-17T00:00:00Z","timestamp":1563321600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","award":["19F1911411"],"award-info":[{"award-number":["19F1911411"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","award":["26106718"],"award-info":[{"award-number":["26106718"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["anatomypubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Developmental Dynamics"],"published-print":{"date-parts":[[2019,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Recent advances in tissue regeneration approaches including 3D organoids, were based on various 3D organogenesis models. However, 3D models are generally technique\u2010sensitive and time\u2010consuming. Thus, we utilized an existing model of submandibular salivary gland (SMG) to modify a simple and highly reproducible in vitro 3D culture model of primary SMG cells self\u2010organization into a well\u2010developed cell spheroid inside Matrigel substrate. We used this model to observe the collective multicellular behavior during spheroid formation. Further, we applied various quantitative approaches including real\u2010time live imaging and immune histochemical image analysis to dissect the cellular dynamics during tissue patterning.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>On a time\u2010scale of hours, we observed marked size and shape transformations in the developed 3D spheroid which resulted in a spatially\u2010controlled growth differential from the canter to the periphery of the formed aggregates. Moreover, we investigated the effect of fibronectin (FN) on SMG cells self\u2010organization using our simplified culture model. Interestingly, we discovered a novel role of FN in inducing duct\u2010like elongation during initial stages of SMG bud formation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This in vitro model provides an excellent tool for analyzing the intercellular dynamics during early SMG tissue development as well as revealing a novel role of FN in SMG ductal expansion.<\/jats:p><\/jats:sec>","DOI":"10.1002\/dvdy.78","type":"journal-article","created":{"date-parts":[[2019,6,25]],"date-time":"2019-06-25T17:30:41Z","timestamp":1561483841000},"page":"813-825","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Fibronectin\u2010induced ductal formation in salivary gland self\u2010organization model"],"prefix":"10.1002","volume":"248","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1085-2405","authenticated-orcid":false,"given":"Mahmoud","family":"Farahat","sequence":"first","affiliation":[{"name":"Department of Biomaterials Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences  Okayama Japan"},{"name":"Department of Oral Rehabilitation and Regenerative Medicine Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences  Okayama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5124-0376","authenticated-orcid":false,"given":"Gulsan A. S.","family":"Kazi","sequence":"additional","affiliation":[{"name":"Department of Biomaterials Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences  Okayama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroaki","family":"Taketa","sequence":"additional","affiliation":[{"name":"Center for the Development of Medical and Health Care Education Okayama University  Okayama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emilio S.","family":"Hara","sequence":"additional","affiliation":[{"name":"Department of Biomaterials Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences  Okayama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masamitsu","family":"Oshima","sequence":"additional","affiliation":[{"name":"Department of Oral Rehabilitation and Regenerative Medicine Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences  Okayama 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