{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T06:27:48Z","timestamp":1778048868853,"version":"3.51.4"},"reference-count":26,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2019,7,26]],"date-time":"2019-07-26T00:00:00Z","timestamp":1564099200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["JP17gm0610004 JP19gm5010001"],"award-info":[{"award-number":["JP17gm0610004 JP19gm5010001"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP26110005 JP16H06385"],"award-info":[{"award-number":["JP26110005 JP16H06385"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["anatomypubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Developmental Dynamics"],"published-print":{"date-parts":[[2019,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The timing of developmental events is tightly regulated along a time axis for normal development. Although the RNA\u2010binding protein Lin28a plays a crucial role in the regulation of developmental timing in <jats:italic>Caenorhabditis elegans<\/jats:italic>, how the timing of Lin28a expression affects the rate and\/or duration of developmental events during mammalian development remains to be addressed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In this study, we discovered that the timing and the duration of <jats:italic>Lin28a<\/jats:italic> expression affect embryonic growth. During the neurulation stage of mouse development, endogenous <jats:italic>Lin28a<\/jats:italic> levels start to drop. When <jats:italic>Lin28a<\/jats:italic> expression was maintained transiently using the inducible tetracycline\u2010regulated gene expression (Tet\u2010ON) system [doxycycline (Dox)\u2010inducible <jats:italic>Lin28a<\/jats:italic> transgenic (<jats:italic>iLin28a<\/jats:italic> Tg) mice] with Dox administration at E8.5 and E9.5, it resulted in neonatal lethality, increased body weight (organomegaly), and an increased number of caudal vertebrae at birth. On the other hand, <jats:italic>Lin28a<\/jats:italic> induction only at E8.5 caused neonatal lethality and organomegaly, but did not affect the caudal vertebra number. Of note, although Dox treatment before or after neurulation still caused neonatal lethality, it neither caused organomegaly nor the increased caudal vertebra number in <jats:italic>iLin28a<\/jats:italic> Tg neonates.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Temporal regulation of Lin28a expression during neurulation affects developmental events such as cessation of axial elongation and embryonic growth in mice.<\/jats:p><\/jats:sec>","DOI":"10.1002\/dvdy.87","type":"journal-article","created":{"date-parts":[[2019,7,13]],"date-time":"2019-07-13T11:33:46Z","timestamp":1563017626000},"page":"931-941","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Temporal regulation of <i>Lin28a<\/i> during mammalian neurulation contributes to neonatal body size 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