{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T18:15:54Z","timestamp":1791051354011,"version":"4.1.0"},"reference-count":27,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2007,1,10]],"date-time":"2007-01-10T00:00:00Z","timestamp":1168387200000},"content-version":"vor","delay-in-days":9,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genesis"],"published-print":{"date-parts":[[2007,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Nonsense\u2010mediated mRNA decay (NMD) is a process of mRNA surveillance that degrades transcripts harboring a premature termination codon (PTC). Mammalian NMD was mostly studied in cultured cells so far and there was no direct evidence yet that NMD could operate in the brain. We introduced, by homologous recombination in mouse, a PTC in the mu opioid receptor gene (<jats:italic>mor<\/jats:italic>). <jats:italic>mor<\/jats:italic> transcript was severely downregulated in the brain of these knock\u2010in mice. A systemic cycloheximide treatment significantly increased the level of the mutant mRNA, suggesting NMD involvement. To further corroborate this hypothesis, we generated a second knock\u2010in mouse line where the PTC was placed at 10 instead of 96 nucleotides from the downstream splice junction. As predicted by the \u201ctermination codon position rule\u201d established in vitro, <jats:italic>mor<\/jats:italic> transcript brain expression was rescued to wild\u2010type level. These knock\u2010in mouse lines will be valuable models to better understand and manipulate NMD in vivo. genesis 45:38\u201343, 2007. \u00a9 2007 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/dvg.20263","type":"journal-article","created":{"date-parts":[[2007,1,10]],"date-time":"2007-01-10T18:48:40Z","timestamp":1168454920000},"page":"38-43","source":"Crossref","is-referenced-by-count":8,"title":["Knock\u2010in mice reveal nonsense\u2010mediated mRNA decay in the brain"],"prefix":"10.1002","volume":"45","author":[{"given":"Candice","family":"Contet","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9e","family":"Dierich","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brigitte L","family":"Kieffer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2007,1,10]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coph.2004.08.012"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.61.3.923"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI7866"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddg054"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb1081"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.48.28995"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M405550200"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/mcb.14.9.6317-6325.1994"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.163.7.2004001"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbrainres.2004.12.022"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceb.2005.04.005"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-437X(03)00014-5"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00109-002-0363-1"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/8.10.1893"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-1004(200001)15:1<45::AID-HUMU10>3.0.CO;2-T"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.01701"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(94)90031-0"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/383819a0"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng1429"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-0004(98)01208-0"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gni052"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.wco.0000162849.37273.af"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.12.4.1009"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.73.011303.073940"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2005.07.002"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa022170"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1017\/S1355838298971849"}],"container-title":["genesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fdvg.20263","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dvg.20263","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,14]],"date-time":"2023-11-14T18:50:32Z","timestamp":1699987832000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/dvg.20263"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,1]]},"references-count":27,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2007,1]]}},"alternative-id":["10.1002\/dvg.20263"],"URL":"https:\/\/doi.org\/10.1002\/dvg.20263","archive":["Portico"],"relation":{},"ISSN":["1526-954X","1526-968X"],"issn-type":[{"value":"1526-954X","type":"print"},{"value":"1526-968X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,1]]}}}