{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,14]],"date-time":"2023-11-14T19:10:06Z","timestamp":1699989006972},"reference-count":19,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2007,2,15]],"date-time":"2007-02-15T00:00:00Z","timestamp":1171497600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genesis"],"published-print":{"date-parts":[[2007,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Extra\u2010toes is a semidominant mutation that affects the <jats:italic>Gli3<\/jats:italic> gene and provokes limb and brain abnormalities. Among the different alleles of this mutation, <jats:italic>Xt<jats:sup>H<\/jats:sup><\/jats:italic> is due to a deletion that has not yet been fully characterized. Using a PCR\u2010based strategy, we undertook a high\u2010resolution mapping of this deletion and confirmed that <jats:italic>Xt<jats:sup>H<\/jats:sup><\/jats:italic> is a null allele of <jats:italic>Gli3<\/jats:italic>. We further designed a PCR test to identify unequivocally heterozygous and homozygous embryos from their wild\u2010type littermates. Despite the length of the <jats:italic>Xt<jats:sup>H<\/jats:sup><\/jats:italic> deletion, available data on the mouse genome indicate that no genes other than <jats:italic>Gli3<\/jats:italic> are deleted in <jats:italic>Xt<jats:sup>H<\/jats:sup><\/jats:italic> mutants. Thus, the <jats:italic>Xt<jats:sup>H<\/jats:sup><\/jats:italic> mutation can be used as a model for studying the effects that absence of Gli3 function has during development. genesis 45:107\u2013112, 2007. \u00a9 2007 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/dvg.20270","type":"journal-article","created":{"date-parts":[[2007,2,15]],"date-time":"2007-02-15T18:18:07Z","timestamp":1171563487000},"page":"107-112","source":"Crossref","is-referenced-by-count":3,"title":["High\u2010resolution mapping of the Gli3 deletion in the mouse extra\u2010toes<sup>H<\/sup> mutant"],"prefix":"10.1002","volume":"45","author":[{"given":"Matthieu","family":"Genestine","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beno\u00eet","family":"Robert","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yvan","family":"Lallemand","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,3,2]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s003359900845"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gki138"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng0393-241"},{"key":"e_1_2_1_5_1","first-page":"543","article-title":"Extra\u2010toes: A new mutant gene causing multiple abnormalities in the mouse","volume":"17","author":"Johnson DR","year":"1967","journal-title":"J Embryol Exp Morphol"},{"key":"e_1_2_1_6_1","first-page":"241","article-title":"GLI3 frameshift mutations cause autosomal dominant Pallister\u2010Hall syndrome","volume":"3","author":"Kang S","year":"1997","journal-title":"Nat Genet"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0016672300010673"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00335-001-2115-X"},{"key":"e_1_2_1_9_1","doi-asserted-by":"crossref","first-page":"1153","DOI":"10.1242\/dev.110.4.1153","article-title":"Evidence for allelism of the recessive insertional mutation add and the dominant mouse syndrome extra\u2010toes (Xt)","volume":"110","author":"Pohl T","year":"1990","journal-title":"Development"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng1197-269"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.10.10.5408"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.116.3.799"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00356560"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s003359900973"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.2144\/00291bm09"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1741-4520.2002.tb00898.x"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00417435"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/352539a0"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00356157"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80678-9"}],"container-title":["genesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fdvg.20270","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dvg.20270","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,14]],"date-time":"2023-11-14T18:52:53Z","timestamp":1699987973000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/dvg.20270"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,3]]},"references-count":19,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2007,3]]}},"alternative-id":["10.1002\/dvg.20270"],"URL":"https:\/\/doi.org\/10.1002\/dvg.20270","archive":["Portico"],"relation":{},"ISSN":["1526-954X","1526-968X"],"issn-type":[{"value":"1526-954X","type":"print"},{"value":"1526-968X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,3]]}}}