{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T06:52:06Z","timestamp":1792651926023,"version":"4.5.0"},"reference-count":16,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2007,1,23]],"date-time":"2007-01-23T00:00:00Z","timestamp":1169510400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genesis"],"published-print":{"date-parts":[[2007,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Tuberous sclerosis complex (TSC) is an autosomal dominant disorder caused by mutations in the <jats:italic>TSC1<\/jats:italic> or <jats:italic>TSC2<\/jats:italic> gene. Patients afflicted with TSC develop tumors in various organ systems, but cerebral pathology is particularly severe. Conventional gene disruption of the <jats:italic>Tsc1<\/jats:italic> or <jats:italic>Tsc2<\/jats:italic> gene in mice cause limited central nervous system pathology. Homozygous deletion of either gene causes midgestation lethality. To circumvent the homozygous lethality of the conventional <jats:italic>Tsc2<\/jats:italic> knockout we have generated a conditional allele of the <jats:italic>Tsc2<\/jats:italic> gene by homologous recombination in mouse ES cells. The homozygous <jats:italic>Tsc2<jats:sup>flox\/flox<\/jats:sup><\/jats:italic> mice are identical to wildtype in many organs typically affected by TSC, especially the brain. Using this <jats:italic>Tsc2<jats:sup>flox<\/jats:sup><\/jats:italic> allele we have generated a null allele using Cre recombination. This allele will be useful in investigating TSC pathology with appropriate cell and organ specific Cre\u2010transgenic mice. genesis 45:101\u2013106, 2007. \u00a9 2007 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/dvg.20271","type":"journal-article","created":{"date-parts":[[2007,1,24]],"date-time":"2007-01-24T00:21:13Z","timestamp":1169598073000},"page":"101-106","source":"Crossref","is-referenced-by-count":93,"title":["Generation of a conditional disruption of the <i>Tsc2<\/i> gene"],"prefix":"10.1002","volume":"45","author":[{"given":"Omar","family":"Hernandez","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sharon","family":"Way","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"III","given":"James","family":"McKenna","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael J.","family":"Gambello","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2007,2,2]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1177\/08830738040190091101"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(93)90618-Z"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/ana.20784"},{"key":"e_1_2_1_5_1","doi-asserted-by":"crossref","DOI":"10.1093\/oso\/9780195122107.001.0001","volume-title":"Tuberous sclerosis complex","author":"Gomez MR","year":"1999"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1001\/archneur.57.5.662"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1528-1167.2005.00289.x"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1203\/01.PDR.0000147727.78571.07"},{"key":"e_1_2_1_9_1","first-page":"1206","article-title":"Renal carcinogenesis, hepatic hemangiomatosis, and embryonic lethality caused by a germ\u2010line Tsc2 mutation in mice","volume":"59","author":"Kobayashi T","year":"1999","journal-title":"Cancer Res"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.151033798"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/11.5.525"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.20357"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI7319"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/75973"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/ana.10283"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.277.5327.805"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddi190"}],"container-title":["genesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fdvg.20271","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dvg.20271","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,15]],"date-time":"2023-11-15T17:34:33Z","timestamp":1700069673000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/dvg.20271"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,2]]},"references-count":16,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2007,2]]}},"alternative-id":["10.1002\/dvg.20271"],"URL":"https:\/\/doi.org\/10.1002\/dvg.20271","archive":["Portico"],"relation":{},"ISSN":["1526-954X","1526-968X"],"issn-type":[{"value":"1526-954X","type":"print"},{"value":"1526-968X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,2]]}}}