{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T19:26:07Z","timestamp":1790796367196,"version":"4.1.0"},"reference-count":27,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2007,10,16]],"date-time":"2007-10-16T00:00:00Z","timestamp":1192492800000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genesis"],"published-print":{"date-parts":[[2007,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Mammalian palate development is a multistep process, involving initial bilateral downward outgrowth of the palatal shelves from the oral side of the maxillary processes, followed by stage\u2010specific palatal shelf elevation to the horizontal position above the developing tongue and subsequent fusion of the bilateral palatal shelves at the midline to form the intact roof of the oral cavity. While mutations in many genes have been associated with cleft palate pathogenesis, the molecular mechanisms regulating palatal shelf growth, patterning, and elevation are not well understood. Genetic studies of the molecular mechanisms controlling palate development in mutant mouse models are often complicated by early embryonic lethality or gross craniofacial malformation. We report here the development of a mouse strain for tissue\u2010specific analysis of gene function in palate development. We inserted an <jats:italic>IresCre<\/jats:italic> bicistronic expression cassette into the 3\u2032 untranslated region of the mouse <jats:italic>Osr2<\/jats:italic> gene through gene targeting. We show, upon crossing to the <jats:italic>R26R<\/jats:italic> reporter mice, that Cre expression from the <jats:italic>Osr2<jats:sup>IresCre<\/jats:sup><\/jats:italic> knockin allele activated \u03b2\u2010galactosidase expression specifically throughout the developing palatal mesenchyme from the onset of palatal shelf outgrowth. In addition, the <jats:italic>Osr2<jats:sup>IresCre<\/jats:sup><\/jats:italic> mice display exclusive Cre\u2010mediated recombination in the glomeruli tissues derived from the metanephric mesenchyme and complete absence of Cre activity in other epithelial and mesenchymal tissues in the developing metanephric kidney. These data indicate that the <jats:italic>Osr2<jats:sup>IresCre<\/jats:sup><\/jats:italic> knockin mice provide a unique tool for tissue\u2010specific studies of the molecular mechanisms regulating palate and kidney development. genesis 45:618\u2013624, 2007. \u00a9 2007 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/dvg.20334","type":"journal-article","created":{"date-parts":[[2007,10,16]],"date-time":"2007-10-16T17:46:52Z","timestamp":1192556812000},"page":"618-624","source":"Crossref","is-referenced-by-count":66,"title":["A unique mouse strain expressing Cre recombinase for tissue\u2010specific analysis of gene function in palate and kidney development"],"prefix":"10.1002","volume":"45","author":[{"given":"Yu","family":"Lan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingru","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine E.","family":"Ovitt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rulang","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2007,10,16]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.127.8.1671"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-8628(19990604)84:4<311::AID-AJMG1>3.0.CO;2-9"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(07)00562-3"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cellbio.22.010305.104340"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/1526-968X(200011\/12)28:3\/4<106::AID-GENE30>3.0.CO;2-T"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.103.Supplement.41"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(93)90619-2"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2006.07.042"},{"key":"e_1_2_1_10_1","volume-title":"Manipulating the mouse embryo: A laboratory manual","author":"Hogan B","year":"1994"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0968-0004(90)90302-R"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.4.9.1560"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(06)00149-7"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0925-4773(01)00457-9"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.01175"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/35093537"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.01676"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.00850"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.9.10.1237"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.12.17.2735"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(93)90620-6"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.124.13.2659"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng0494-348"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.14.5166"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/5007"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.7.11.2071"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1177\/154411130301400302"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.8.3789"}],"container-title":["genesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fdvg.20334","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dvg.20334","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,14]],"date-time":"2023-11-14T10:48:46Z","timestamp":1699958926000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/dvg.20334"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,10]]},"references-count":27,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2007,10]]}},"alternative-id":["10.1002\/dvg.20334"],"URL":"https:\/\/doi.org\/10.1002\/dvg.20334","archive":["Portico"],"relation":{},"ISSN":["1526-954X","1526-968X"],"issn-type":[{"value":"1526-954X","type":"print"},{"value":"1526-968X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,10]]}}}