{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T06:29:16Z","timestamp":1780640956571,"version":"3.54.1"},"reference-count":19,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2008,9,18]],"date-time":"2008-09-18T00:00:00Z","timestamp":1221696000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genesis"],"published-print":{"date-parts":[[2008,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Eph receptor tyrosine kinases (RTKs) are a highly conserved family of signaling proteins with functions in cellular migration, adhesion, apoptosis, and proliferation during both adult and embryonic life. Here, we describe a knock\u2010in mouse in which <jats:italic>EphA1<\/jats:italic> expression is disrupted via the insertion of an internal ribosome entry site (IRES)\u2010human placental alkaline phosphatase (ALPP) reporter cassette into exon II of the <jats:italic>EphA1<\/jats:italic> gene. This was shown to successfully knockout expression of endogenous <jats:italic>EphA1<\/jats:italic> and enforce expression of the ALPP reporter by the <jats:italic>EphA1<\/jats:italic> promoter. Staining for the ALPP reporter protein demonstrated an epithelially restricted expression pattern in mouse tissues. In <jats:italic>EphA1<\/jats:italic> null mice, two separate phenotypes were identified: abnormal tail development manifesting as a kinky tail was found in \u223c80% of homozygous adults. A second, distinct abnormality present in \u223c18% of females was characterized by imperforate uterovaginal development with hydrometrocolpos and caused by a resistance of cells to apoptosis during reproductive tract canalization. These results indicate a possible role for EphA1 in tissue patterning and hormone\u2010induced apoptotic processes. genesis 46:553\u2013561, 2008. \u00a9 2008 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/dvg.20434","type":"journal-article","created":{"date-parts":[[2008,9,18]],"date-time":"2008-09-18T22:18:14Z","timestamp":1221776294000},"page":"553-561","source":"Crossref","is-referenced-by-count":30,"title":["Generation and characterization of <i>EphA1<\/i> receptor tyrosine kinase reporter knockout mice"],"prefix":"10.1002","volume":"46","author":[{"given":"Shannon L.","family":"Duffy","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark G.","family":"Coulthard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark D.","family":"Spanevello","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nirmitha 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