{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,9]],"date-time":"2026-05-09T05:07:21Z","timestamp":1778303241221,"version":"3.51.4"},"reference-count":20,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2008,12,29]],"date-time":"2008-12-29T00:00:00Z","timestamp":1230508800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genesis"],"published-print":{"date-parts":[[2009,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The epidermal growth factor receptor (EGFR) is important for normal homeostasis in a variety of tissues and, when abnormally expressed or mutated, contributes to the development of many diseases. However, in vivo functional studies are hindered by the lack of adult mice lacking EGFR because of the pre\u2010 and postnatal lethality of EGFR deficient mice. We generated a conditional allele of <jats:italic>Egfr<\/jats:italic> (<jats:italic>Egfr<\/jats:italic><jats:sup><jats:italic>tm1Dwt<\/jats:italic><\/jats:sup>) by flanking exon 3 with <jats:italic>loxP<\/jats:italic> sites in order to investigate tissue\u2010specific functions of this widely expressed receptor tyrosine kinase. The activity of the <jats:italic>Egfr<\/jats:italic><jats:sup><jats:italic>tm1Dwt<\/jats:italic><\/jats:sup> allele is indistinguishable from wildtype <jats:italic>Egfr<\/jats:italic>. Conversely, the <jats:italic>Egfr<jats:sup>\u0394<\/jats:sup><\/jats:italic> allele, generated by Cre\u2010mediated deletion of exon 3 using the germline <jats:italic>EIIa\u2010Cre<\/jats:italic> transgenic line, functions as a null allele. <jats:italic>Egfr<jats:sup>\u0394\/\u0394<\/jats:sup><\/jats:italic> embryos that have complete ablation of EGFR activity and die at mid\u2010gestation with placental defects identical to those reported for mice homozygous for the <jats:italic>Egfr<\/jats:italic><jats:sup><jats:italic>tm1Mag<\/jats:italic><\/jats:sup> null allele. We also inactivated the <jats:italic>Egfr<\/jats:italic><jats:sup><jats:italic>tm1Dwt<\/jats:italic><\/jats:sup> allele tissue\u2010specifically in the skin epithelium using the <jats:italic>K14\u2010Cre<\/jats:italic> transgenic line. These mice were viable but exhibited wavy coat hair remarkably similar to mice homozygous for the <jats:italic>Egfr<\/jats:italic><jats:sup><jats:italic>wa2<\/jats:italic><\/jats:sup> hypomorphic allele or heterozygous for the <jats:italic>Egfr<\/jats:italic><jats:sup><jats:italic>Wa5<\/jats:italic><\/jats:sup> antimorphic allele. These results suggest that the hairless phenotype of <jats:italic>Egfr<\/jats:italic> nullizygous mice is not solely due to absence of EGFR in the epithelium, but that EGFR activity is required also in skin stromal cells for normal hair morphogenesis. This new mouse model should have wide utility to inactivate <jats:italic>Egfr<\/jats:italic> conditionally for functional analysis of EGFR in adult tissues and disease states. genesis 47:85\u201392, 2009. \u00a9 2008 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/dvg.20464","type":"journal-article","created":{"date-parts":[[2008,12,31]],"date-time":"2008-12-31T19:34:50Z","timestamp":1230752090000},"page":"85-92","source":"Crossref","is-referenced-by-count":96,"title":["Generation and validation of mice carrying a conditional allele of the epidermal growth factor receptor"],"prefix":"10.1002","volume":"47","author":[{"given":"Tang\u2010Cheng","family":"Lee","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David W.","family":"Threadgill","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,12,29]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/73528"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm0502-459"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.placenta.2007.07.005"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.127.22.4775"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.5.1465"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(02)00940-6"},{"key":"e_1_2_1_8_1","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1093\/oxfordjournals.jhered.a104066","article-title":"A second rexoid coat character in the house mouse","volume":"26","author":"Keeler CE","year":"1935","journal-title":"J Hered"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.12.5860"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.00715"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.8.4.399"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M212082200"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/376337a0"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/8773"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0704126104"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvg.20243"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1006\/geno.2000.6341"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.00664"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.7618085"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.103.020495"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.7618084"}],"container-title":["genesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fdvg.20464","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dvg.20464","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,13]],"date-time":"2023-11-13T17:52:28Z","timestamp":1699897948000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/dvg.20464"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,12,29]]},"references-count":20,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2009,2]]}},"alternative-id":["10.1002\/dvg.20464"],"URL":"https:\/\/doi.org\/10.1002\/dvg.20464","archive":["Portico"],"relation":{},"ISSN":["1526-954X","1526-968X"],"issn-type":[{"value":"1526-954X","type":"print"},{"value":"1526-968X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,12,29]]}}}