{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T04:19:08Z","timestamp":1788236348343,"version":"build-2803163510"},"reference-count":13,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2009,6,22]],"date-time":"2009-06-22T00:00:00Z","timestamp":1245628800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genesis"],"published-print":{"date-parts":[[2009,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Glucose\u20106\u2010phosphatase\u2010\u03b1 (G6Pase\u2010\u03b1 or G6PC) catalyzes the hydrolysis of glucose\u20106\u2010phosphate to glucose and is a key enzyme in interprandial glucose homeostasis. Mutations in the human <jats:italic>G6PC<\/jats:italic> gene, expressed primarily in the liver, kidney, and intestine, cause glycogen storage disease Type Ia (GSD\u2010Ia), an autosomal recessive disorder characterized by a disturbed glucose homeostasis. For better understanding of the roles of G6Pase\u2010\u03b1 in different tissues and in pathological conditions, we have generated mice harboring a conditional null allele for <jats:italic>G6pc<\/jats:italic> by flanking Exon 3 of the <jats:italic>G6pc<\/jats:italic> gene with <jats:italic>loxP<\/jats:italic> sites. We confirmed the null phenotype by using the <jats:italic>EIIa\u2010Cre<\/jats:italic> transgenic approach to generate mice lacking Exon 3 of the <jats:italic>G6pc<\/jats:italic> gene. The resulting homozygous <jats:italic>Cre<\/jats:italic>\u2010recombined null mice manifest a phenotype mimicking G6Pase\u2010\u03b1\u2010deficient mice and human GSD\u2010Ia patients. This <jats:italic>G6pc<\/jats:italic> conditional null allele will be valuable to examine the consequence of tissue\u2010specific G6Pase\u2010\u03b1 deficiency and the mechanisms of long\u2010term complications in GSD\u2010Ia. genesis 47:590\u2013594, 2009. Published 2009 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/dvg.20538","type":"journal-article","created":{"date-parts":[[2009,6,22]],"date-time":"2009-06-22T16:48:10Z","timestamp":1245689290000},"page":"590-594","source":"Crossref","is-referenced-by-count":19,"title":["Generation of mice with a conditional allele for G6pc"],"prefix":"10.1002","volume":"47","author":[{"given":"Wen\u2010Tao","family":"Peng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chi\u2010Jiunn","family":"Pan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric J.","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heiner","family":"Westphal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janice Y.","family":"Chou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,6,22]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198401193100306"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.2174\/1566524024605798"},{"key":"e_1_2_1_4_1","doi-asserted-by":"crossref","first-page":"1136","DOI":"10.2144\/03346bm03","article-title":"Optimized vector for conditional gene targeting in mouse embryonic stem cells","volume":"34","author":"Conrad M","year":"2003","journal-title":"Biotechniques"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.50.4.740"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/1526-968X(200011\/12)28:3\/4<106::AID-GENE30>3.0.CO;2-T"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197602192940805"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02679995"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.12.5860"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng0696-203"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/336348a0"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/PL00014320"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1006\/abbi.1998.0849"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1016572706488"}],"container-title":["genesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fdvg.20538","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dvg.20538","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,15]],"date-time":"2023-11-15T14:57:53Z","timestamp":1700060273000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/dvg.20538"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,6,22]]},"references-count":13,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2009,9]]}},"alternative-id":["10.1002\/dvg.20538"],"URL":"https:\/\/doi.org\/10.1002\/dvg.20538","archive":["Portico"],"relation":{},"ISSN":["1526-954X","1526-968X"],"issn-type":[{"value":"1526-954X","type":"print"},{"value":"1526-968X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,6,22]]}}}