{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,16]],"date-time":"2026-04-16T07:14:50Z","timestamp":1776323690223,"version":"3.50.1"},"reference-count":51,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2009,12,15]],"date-time":"2009-12-15T00:00:00Z","timestamp":1260835200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genesis"],"published-print":{"date-parts":[[2010,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Nodal\u2010signaling is required for specification of mesoderm, endoderm, establishing left\u2013right asymmetry, and craniofacial development. Wdr68 is a WD40\u2010repeat domain\u2010containing protein recently shown to be required for <jats:italic>endothelin\u20101 (edn1<\/jats:italic>) expression and subsequent lower jaw development. Previous reports detected the Wdr68 protein in multiprotein complexes containing mammalian members of the <jats:italic>dual\u2010specificity tyrosine\u2010regulated kinase (dyrk<\/jats:italic>) family. Here we describe the characterization of the zebrafish <jats:italic>dyrk1b<\/jats:italic> homolog. We report the detection of a physical interaction between Dyrk1b and Wdr68. We also found perturbations of nodal signaling in <jats:italic>dyrk1b<\/jats:italic> antisense morpholino knockdown (<jats:italic>dyrk1b\u2010<\/jats:italic>MO) animals. Specifically, we found reduced expression of <jats:italic>lft1<\/jats:italic> and <jats:italic>lft2<\/jats:italic> (<jats:italic>lft1\/2<\/jats:italic>) during gastrulation and a near complete loss of the later asymmetric <jats:italic>lft1<\/jats:italic>\/<jats:italic>2<\/jats:italic> expression domains. Although <jats:italic>wdr68<\/jats:italic>\u2010MO animals did not display <jats:italic>lft1\/2<\/jats:italic> expression defects during gastrulation, they displayed a near complete loss of the later asymmetric <jats:italic>lft1<\/jats:italic>\/<jats:italic>2<\/jats:italic> expression domains. While expression of <jats:italic>ndr1<\/jats:italic> was not substantially effected during gastrulation, <jats:italic>ndr2<\/jats:italic> expression was moderately reduced in <jats:italic>dyrk1b\u2010<\/jats:italic>MO animals. Analysis of additional downstream components of the nodal signaling pathway in <jats:italic>dyrk1b\u2010<\/jats:italic>MO animals revealed modestly expanded expression of the dorsal axial mesoderm marker <jats:italic>gsc<\/jats:italic> while the pan\u2010mesodermal marker <jats:italic>bik<\/jats:italic> was largely unaffected. The endodermal markers <jats:italic>cas<\/jats:italic> and <jats:italic>sox17<\/jats:italic> were also moderately reduced in <jats:italic>dyrk1b\u2010<\/jats:italic>MO animals. Notably, and similar to defects previously reported for <jats:italic>wdr68<\/jats:italic> mutant animals, we also found reduced expression of the pharyngeal pouch marker <jats:italic>edn1<\/jats:italic> in <jats:italic>dyrk1b\u2010<\/jats:italic>MO animals. Taken together, these data reveal a role for <jats:italic>dyrk1b<\/jats:italic> in endoderm formation and craniofacial patterning in the zebrafish. genesis 48:20\u201330, 2010. \u00a9 2009 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/dvg.20578","type":"journal-article","created":{"date-parts":[[2009,12,15]],"date-time":"2009-12-15T17:14:59Z","timestamp":1260897299000},"page":"20-30","source":"Crossref","is-referenced-by-count":27,"title":["The zebrafish <i>dyrk1b<\/i> gene is important for endoderm formation"],"prefix":"10.1002","volume":"48","author":[{"given":"Gohar","family":"Mazmanian","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Kovshilovsky","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Debbie","family":"Yen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aditya","family":"Mohanty","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sudipta","family":"Mohanty","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alex","family":"Nee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert 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