{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T05:34:45Z","timestamp":1783402485658,"version":"3.54.6"},"reference-count":26,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2010,8,12]],"date-time":"2010-08-12T00:00:00Z","timestamp":1281571200000},"content-version":"vor","delay-in-days":36,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genesis"],"published-print":{"date-parts":[[2010,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The Wnt\u2010signaling pathway is necessary in a variety of developmental processes and has been implicated in numerous pathologies. Wntless (Wls) binds to Wnt proteins and facilitates Wnt sorting and secretion. Conventional deletion of <jats:italic>Wls<\/jats:italic> results in early fetal lethality due to defects in body axis establishment. To gain insight into the function of <jats:italic>Wls<\/jats:italic> in later stages of development, we have generated a conditional null allele. Homozygous germline deletion of <jats:italic>Wls<\/jats:italic> confirmed prenatal lethality and failure of embryonic axis formation. Deletion of <jats:italic>Wls<\/jats:italic> using <jats:italic>Wnt1\u2010cre<\/jats:italic> phenocopied Wnt1 null abnormalities in the midbrain and hindbrain. In addition, conditional deletion of <jats:italic>Wls<\/jats:italic> in pancreatic precursor cells resulted in pancreatic hypoplasia similar to that previously observed after conditional \u03b2\u2010<jats:italic>catenin<\/jats:italic> deletion. This <jats:italic>Wls<\/jats:italic> conditional null allele will be valuable in detecting novel Wnt functions in development and disease. genesis 48:554\u2013558, 2010. \u00a9 2010 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/dvg.20651","type":"journal-article","created":{"date-parts":[[2010,7,7]],"date-time":"2010-07-07T19:39:45Z","timestamp":1278531585000},"page":"554-558","source":"Crossref","is-referenced-by-count":152,"title":["Generation of mice with a conditional null allele for <i>Wntless<\/i>"],"prefix":"10.1002","volume":"48","author":[{"given":"April C.","family":"Carpenter","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sujata","family":"Rao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James M.","family":"Wells","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenneth","family":"Campbell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard A.","family":"Lang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2010,7,7]]},"reference":[{"key":"e_1_2_4_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2006.02.049"},{"key":"e_1_2_4_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2006.04.009"},{"key":"e_1_2_4_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2007.12.003"},{"key":"e_1_2_4_5_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.128.8.1253"},{"key":"e_1_2_4_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(07)00562-3"},{"key":"e_1_2_4_7_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.126.20.4557"},{"key":"e_1_2_4_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2005.08.037"},{"key":"e_1_2_4_9_1","doi-asserted-by":"crossref","first-page":"2213","DOI":"10.1242\/dev.120.8.2213","article-title":"Cis\u2010acting regulatory sequences governing Wnt\u20101 expression in the developing mouse CNS","volume":"120","author":"Echelard Y","year":"1994","journal-title":"Development"},{"key":"e_1_2_4_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1678"},{"key":"e_1_2_4_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0904894106"},{"key":"e_1_2_4_12_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.02674"},{"key":"e_1_2_4_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.10157"},{"key":"e_1_2_4_14_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.148.3.567"},{"key":"e_1_2_4_15_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.2002.00929.x"},{"key":"e_1_2_4_16_1","doi-asserted-by":"publisher","DOI":"10.1186\/1741-7007-3-23"},{"key":"e_1_2_4_17_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.12.5860"},{"key":"e_1_2_4_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/11932"},{"key":"e_1_2_4_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(90)90385-R"},{"key":"e_1_2_4_20_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.02063"},{"key":"e_1_2_4_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2007.12.001"},{"key":"e_1_2_4_22_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.119.1.247"},{"key":"e_1_2_4_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1687"},{"key":"e_1_2_4_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/75973"},{"key":"e_1_2_4_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2009.05.012"},{"key":"e_1_2_4_26_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-213X-7-4"},{"key":"e_1_2_4_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2007.12.004"}],"container-title":["genesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fdvg.20651","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dvg.20651","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,21]],"date-time":"2023-11-21T06:46:27Z","timestamp":1700549187000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/dvg.20651"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,7,7]]},"references-count":26,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2010,9]]}},"alternative-id":["10.1002\/dvg.20651"],"URL":"https:\/\/doi.org\/10.1002\/dvg.20651","archive":["Portico"],"relation":{},"ISSN":["1526-954X","1526-968X"],"issn-type":[{"value":"1526-954X","type":"print"},{"value":"1526-968X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,7,7]]}}}