{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T14:06:24Z","timestamp":1761919584072},"reference-count":13,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2012,5,19]],"date-time":"2012-05-19T00:00:00Z","timestamp":1337385600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genesis"],"published-print":{"date-parts":[[2012,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Crim1 is a developmentally expressed, transmembrane protein essential for normal embryonic development. We generated mice engineered to contain a <jats:italic>Crim1<\/jats:italic> conditional null allele by flanking exons three and four of <jats:italic>Crim1<\/jats:italic> with unidirectional LoxP sites. After crossing <jats:italic>Crim1<jats:sup>+\/FLOX<\/jats:sup><\/jats:italic> mice with a CMV\u2010Cre line, a <jats:italic>Crim1<jats:sup>+\/\u0394flox<\/jats:sup><\/jats:italic> colony was established after germline transmission of the deleted allele. We then analyzed genomic DNA, mRNA transcripts, and protein expression from <jats:italic>Crim1<jats:sup>\u0394flox\/\u0394flox<\/jats:sup><\/jats:italic> null mice to confirm the nature of the genomic lesion. <jats:italic>Crim1<jats:sup>\u0394flox\/\u0394flox<\/jats:sup><\/jats:italic> mice displayed phenotypes similar to those previously described for a <jats:italic>Crim1<\/jats:italic> gene\u2010trap mutant, <jats:italic>Crim1<jats:sup>KST264\/KST264<\/jats:sup><\/jats:italic>, including perinatal lethality, digit syndactyly, eye, and kidney abnormalities, with varying penetrance and severity. The production of a conditional mutant allele represents a valuable resource for the study of the tissue\u2010specific roles for <jats:italic>Crim1<\/jats:italic>, and for understanding the pleimorphic phenotypes associated with <jats:italic>Crim1<\/jats:italic> mutation. genesis 50:711\u2013716, 2012. \u00a9 2012 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/dvg.22032","type":"journal-article","created":{"date-parts":[[2012,4,17]],"date-time":"2012-04-17T13:48:44Z","timestamp":1334670524000},"page":"711-716","source":"Crossref","is-referenced-by-count":15,"title":["Production of a mouse line with a conditional <i>Crim1<\/i> mutant allele"],"prefix":"10.1002","volume":"50","author":[{"given":"Han Sheng","family":"Chiu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. Philippe","family":"York","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lorine","family":"Wilkinson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pumin","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Melissa H.","family":"Little","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David J.","family":"Pennisi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,5,19]]},"reference":[{"key":"e_1_2_4_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0177(2000)9999:9999<::AID-DVDY1072>3.0.CO;2-I"},{"key":"e_1_2_4_3_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.066142"},{"key":"e_1_2_4_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0925-4773(99)00248-8"},{"key":"e_1_2_4_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/ndt\/gfn743"},{"key":"e_1_2_4_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.placenta.2011.12.014"},{"key":"e_1_2_4_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2009.01.023"},{"key":"e_1_2_4_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.21015"},{"key":"e_1_2_4_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/gene.10043"},{"key":"e_1_2_4_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.26.14984"},{"key":"e_1_2_4_11_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2006091012"},{"key":"e_1_2_4_12_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M301247200"},{"key":"e_1_2_4_13_1","doi-asserted-by":"publisher","DOI":"10.4161\/rna.8.5.16043"},{"key":"e_1_2_4_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng797"}],"container-title":["genesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fdvg.22032","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dvg.22032","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,21]],"date-time":"2023-11-21T07:28:51Z","timestamp":1700551731000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/dvg.22032"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,5,19]]},"references-count":13,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2012,9]]}},"alternative-id":["10.1002\/dvg.22032"],"URL":"https:\/\/doi.org\/10.1002\/dvg.22032","archive":["Portico"],"relation":{},"ISSN":["1526-954X","1526-968X"],"issn-type":[{"value":"1526-954X","type":"print"},{"value":"1526-968X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,5,19]]}}}