{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T17:39:55Z","timestamp":1792172395194,"version":"4.4.0"},"reference-count":29,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2012,6,19]],"date-time":"2012-06-19T00:00:00Z","timestamp":1340064000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genesis"],"published-print":{"date-parts":[[2012,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Summary: R\u2010spondins are secreted ligands that bind cell surface receptors and activate Wnt\/\u03b2\u2010catenin signaling. Human mutations and gene inactivation studies in mice have revealed a role for these four proteins (RSPO1\u20104) in diverse developmental processes ranging from sex determination to limb development. Among the genes coding for R\u2010spondins, only inactivation of <jats:italic>Rspo3<\/jats:italic> shows early embryonic lethality (E10.5 in mice). Therefore, a conditional allele of this gene is necessary to understand the function of R\u2010spondins throughout murine development. To address this need, we have produced an allele in which <jats:italic>loxP<\/jats:italic> sites flank exons 2\u20134 of <jats:italic>Rspo3<\/jats:italic>, allowing tissue\u2010specific deletion of these exons in the presence of Cre recombinase. We used these mice to investigate the role of <jats:italic>Rspo3<\/jats:italic> during limb development and found that limbs ultimately developed normally in the absence of <jats:italic>Rspo3<\/jats:italic> function. However, severe hindlimb truncations resulted when <jats:italic>Rspo3<\/jats:italic> and <jats:italic>Rspo2<\/jats:italic> mutations were combined, demonstrating redundant function of these genes. genesis 50:741\u2013749, 2012. \u00a9 2012 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/dvg.22040","type":"journal-article","created":{"date-parts":[[2012,5,18]],"date-time":"2012-05-18T12:19:40Z","timestamp":1337343580000},"page":"741-749","source":"Crossref","is-referenced-by-count":42,"title":["A conditional allele of <i>Rspo3<\/i> reveals redundant function of R\u2010spondins during mouse limb development"],"prefix":"10.1002","volume":"50","author":[{"given":"Stanley","family":"Neufeld","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica 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