{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T09:42:14Z","timestamp":1789897334975,"version":"4.0.1"},"reference-count":32,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2012,8,21]],"date-time":"2012-08-21T00:00:00Z","timestamp":1345507200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genesis"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Nonsyndromic cleft lip and palate (NSCLP), a common birth defect, affects 4,000 newborns in the US each year. Previously, we described an association between <jats:italic>CRISPLD2<\/jats:italic> and NSCLP and showed <jats:italic>Crispld2<\/jats:italic> expression in the murine palate. These results suggested that a perturbation in CRISPLD2 activity affects craniofacial development. Here, we describe <jats:italic>crispld2<\/jats:italic> expression and the phenotypic consequence of its loss of function in zebrafish. <jats:italic>crispld2<\/jats:italic> was expressed at all stages of zebrafish morphogenesis examined and localized to the rostral end by 1\u2010day postfertilization. Morpholino knockdown of <jats:italic>crispld2<\/jats:italic> resulted in significant jaw and palatal abnormalities in a dose\u2010dependent manner. Loss of <jats:italic>crispld2<\/jats:italic> caused aberrant patterning of neural crest cells (NCC) suggesting that <jats:italic>crispld2<\/jats:italic> is necessary for normal NCC formation. Altogether, we show that <jats:italic>crispld2<\/jats:italic> plays a significant role in the development of the zebrafish craniofacies and alteration of normal protein levels disturbs palate and jaw formation. These data provide support for a role of CRISPLD2 in NSCLP. genesis 50:871\u2013881, 2012. \u00a9 2012 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/dvg.22051","type":"journal-article","created":{"date-parts":[[2012,8,6]],"date-time":"2012-08-06T07:03:09Z","timestamp":1344236589000},"page":"871-881","source":"Crossref","is-referenced-by-count":30,"title":["Craniofacial abnormalities result from knock down of nonsyndromic clefting gene, <i>crispld2<\/i>, in zebrafish"],"prefix":"10.1002","volume":"50","author":[{"given":"Qiuping","family":"Yuan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brett T.","family":"Chiquet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"DeVault","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew 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