{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T20:58:50Z","timestamp":1791147530259,"version":"4.1.0"},"reference-count":23,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2012,9,12]],"date-time":"2012-09-12T00:00:00Z","timestamp":1347408000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genesis"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>After thymic emigration CD4\u2010T\u2010cells continue to differentiate into multiple effector and suppressor sublineages in peripheral lymphoid organs. <jats:italic>In vivo<\/jats:italic> analysis of peripheral CD4\u2010T\u2010cell differentiation has relied on animal models with targeted gene mutations. These are expressed either constitutively or conditionally after Cre mediated recombination. Available Cre transgenic strains to specifically target T\u2010cells act at stages of thymocyte development that precede thymic selection. Tracing gene functions in CD4\u2010T\u2010cell development after thymic exit becomes complicated when the targeted gene is essential during thymic development. Other approaches to conditionally modify gene functions in peripheral T\u2010cells involve infection of <jats:italic>in vitro<\/jats:italic> activated cells with Cre expressing lenti\u2010, retro\u2010, or adenoviruses, which precludes <jats:italic>in vivo<\/jats:italic> analyses. To study molecular mechanisms of peripheral CD4\u2010T\u2010cell differentiation <jats:italic>in vivo<\/jats:italic> and <jats:italic>in vitro<\/jats:italic> we generated transgenic mice expressing a tamoxifen inducible Cre recombinase (CreER<jats:sup>T2<\/jats:sup>) under the control of the CD4 gene promoter. We show here that in CD4CreER<jats:sup>T2<\/jats:sup> mice Cre is inducibly and selectively activated in CD4\u2010T\u2010cells. Tamoxifen treatment both <jats:italic>in vivo<\/jats:italic> and <jats:italic>in vitro<\/jats:italic> results in efficient recombination of loci marked by LoxP sites. Moreover, this strain shows no abnormalities related to transgene insertion. Therefore it provides a valuable tool for studying gene function during differentiation of na\u00efve peripheral CD4\u2010T\u2010cells into effector or suppressor sub\u2010lineages. genesis 50:908\u2013913, 2012. \u00a9 2012 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/dvg.22052","type":"journal-article","created":{"date-parts":[[2012,8,10]],"date-time":"2012-08-10T11:28:52Z","timestamp":1344598132000},"page":"908-913","source":"Crossref","is-referenced-by-count":85,"title":["Generation of CD4CreER<sup>T2<\/sup> transgenic mice to study development of peripheral CD4\u2010T\u2010cells"],"prefix":"10.1002","volume":"50","author":[{"given":"Katayoun","family":"Aghajani","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shilpa","family":"Keerthivasan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fotini","family":"Gounari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,9,12]]},"reference":[{"key":"e_1_2_4_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.161272698"},{"key":"e_1_2_4_3_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1997.7124"},{"key":"e_1_2_4_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni778"},{"key":"e_1_2_4_5_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2006-11-059071"},{"key":"e_1_2_4_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/18.21.5931"},{"key":"e_1_2_4_7_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.2002.0597"},{"key":"e_1_2_4_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.26.12070"},{"key":"e_1_2_4_9_1","doi-asserted-by":"publisher","DOI":"10.1385\/IR:26:1-3:131"},{"key":"e_1_2_4_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/27.22.4324"},{"key":"e_1_2_4_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature10772"},{"key":"e_1_2_4_12_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1998.2307"},{"key":"e_1_2_4_13_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0901369"},{"key":"e_1_2_4_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(01)00227-8"},{"key":"e_1_2_4_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1044-7431(03)00029-0"},{"key":"e_1_2_4_16_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20081062"},{"key":"e_1_2_4_17_1","first-page":"379","article-title":"Targeted conditional somatic mutagenesis in the mouse: Temporally controlled knock out of retinoid receptors in epidermal keratinocytes","volume":"364","author":"Metzger D","year":"2003","journal-title":"Methods Enzymol"},{"key":"e_1_2_4_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri954"},{"key":"e_1_2_4_19_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.201415498"},{"key":"e_1_2_4_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/29.16.e80"},{"key":"e_1_2_4_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(93)25056-8"},{"key":"e_1_2_4_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni1387"},{"key":"e_1_2_4_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/5007"},{"key":"e_1_2_4_24_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-213X-1-4"}],"container-title":["genesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fdvg.22052","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dvg.22052","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,21]],"date-time":"2023-11-21T16:23:46Z","timestamp":1700583826000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/dvg.22052"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,9,12]]},"references-count":23,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2012,12]]}},"alternative-id":["10.1002\/dvg.22052"],"URL":"https:\/\/doi.org\/10.1002\/dvg.22052","archive":["Portico"],"relation":{},"ISSN":["1526-954X","1526-968X"],"issn-type":[{"value":"1526-954X","type":"print"},{"value":"1526-968X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,9,12]]}}}