{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T03:35:17Z","timestamp":1761708917401},"reference-count":26,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2013,9,2]],"date-time":"2013-09-02T00:00:00Z","timestamp":1378080000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"NICHD","award":["R01-30428","R01-34283"],"award-info":[{"award-number":["R01-30428","R01-34283"]}]},{"name":"International Endocrine Scholar Program (MIPM)"},{"name":"Transgenic Animal Model Core"},{"name":"NIH","award":["CA46592","AR20557","DK34933"],"award-info":[{"award-number":["CA46592","AR20557","DK34933"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genesis"],"published-print":{"date-parts":[[2013,11]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Tissue\u2010specific expression of <jats:italic>cre<\/jats:italic> recombinase is a well\u2010established genetic tool to analyze gene function, and it is limited only by the efficiency and specificity of available <jats:italic>cre<\/jats:italic> mouse strains. Here, we report the generation of a transgenic line containing a <jats:italic>cre<\/jats:italic> cassette with codon usage optimized for mammalian cells (iCre) under the control of a mouse glycoprotein hormone \u03b1\u2010subunit (\u03b1GSU) regulatory sequences in a bacterial artificial chromosome genomic clone. Initial analysis of this transgenic line, <jats:italic>Tg(\u03b1GSU\u2010iCre)<\/jats:italic>, with <jats:italic>cre<\/jats:italic> reporter strains reveals onset of <jats:italic>cre<\/jats:italic> activity in the differentiating cells of the developing anterior pituitary gland at embryonic day 12.5, with a pattern characteristic of endogenous \u03b1GSU. In adult mice, \u03b1<jats:italic>GSU\u2010iCre<\/jats:italic> was active in the anterior lobe of the pituitary gland and in the cells that produce \u03b1GSU (gonadotropes and thyrotropes) with high penetrance. Little or no activity was observed in other tissues, including skeletal and cardiac muscle, brain, kidney, lungs, testis, ovary, and liver. This \u03b1<jats:italic>GSU\u2010iCre<\/jats:italic> line is suitable for efficient, specific, and developmentally regulated deletion of floxed alleles in anterior pituitary gonadotropes and thyrotropes. genesis 51:785\u2013792. \u00a9 2013 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/dvg.22425","type":"journal-article","created":{"date-parts":[[2013,8,12]],"date-time":"2013-08-12T16:08:50Z","timestamp":1376323730000},"page":"785-792","source":"Crossref","is-referenced-by-count":15,"title":["Efficient, specific, developmentally appropriate cre\u2010mediated recombination in anterior pituitary gonadotropes and thyrotropes"],"prefix":"10.1002","volume":"51","author":[{"given":"Mar\u00eda In\u00e9s","family":"P\u00e9rez\u2010Mill\u00e1n","sequence":"first","affiliation":[{"name":"Department of Human Genetics University of Michigan Ann Arbor Michigan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael G.","family":"Zeidler","sequence":"additional","affiliation":[{"name":"Department of Human Genetics University of Michigan Ann Arbor Michigan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas L.","family":"Saunders","sequence":"additional","affiliation":[{"name":"Department of Human Genetics University of Michigan Ann Arbor Michigan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sally A.","family":"Camper","sequence":"additional","affiliation":[{"name":"Department of Human Genetics University of Michigan Ann Arbor Michigan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shannon W.","family":"Davis","sequence":"additional","affiliation":[{"name":"Department of Human Genetics University of Michigan Ann Arbor Michigan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,9,2]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1988.tb02824.x"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1210\/me.2009-0030"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1210\/me.12.5.622"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1105929108"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1210\/me.10.11.1467"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1210\/me.2005-0378"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1043-2760(05)80007-4"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/1526-968X(200011\/12)28:3\/4<167::AID-GENE120>3.0.CO;2-N"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2011.01.010"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.5.9.1513"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1177\/42.8.8027530"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1210\/me.8.10.1420"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1210\/mend-5-12-2025"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1006\/geno.2000.6451"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn.2467"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.00192"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvg.20335"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1530\/eje.1.02326"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2826.2002.00808.x"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/gene.10023"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/5007"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2009.11.021"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11248-009-9271-2"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.8.3789"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1006\/geno.2001.6616"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gde.2004.08.006"}],"container-title":["genesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fdvg.22425","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dvg.22425","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,7]],"date-time":"2023-10-07T01:08:08Z","timestamp":1696640888000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/dvg.22425"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,9,2]]},"references-count":26,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2013,11]]}},"alternative-id":["10.1002\/dvg.22425"],"URL":"https:\/\/doi.org\/10.1002\/dvg.22425","archive":["Portico"],"relation":{},"ISSN":["1526-954X","1526-968X"],"issn-type":[{"value":"1526-954X","type":"print"},{"value":"1526-968X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,9,2]]}}}