{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T06:53:27Z","timestamp":1791269607471,"version":"4.1.0"},"reference-count":31,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2013,11,21]],"date-time":"2013-11-21T00:00:00Z","timestamp":1384992000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genesis"],"published-print":{"date-parts":[[2013,12]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    Gene inactivation is an important tool for correlation of phenotypic and genomic data, allowing researchers to infer normal gene function based on the phenotype when the gene is impaired. New and better approaches are needed to overcome the shortfalls of existing methods for any significant acceleration of scientific progress. We have adapted the CRISPR\/Cas system for use in\n                    <jats:italic>Xenopus tropicalis<\/jats:italic>\n                    and report on the efficient creation of mutations in the gene encoding the enzyme tyrosinase, which is responsible for oculocutaneous albinism. Biallelic mutation of this gene was detected in the F\n                    <jats:sub>0<\/jats:sub>\n                    generation, suggesting targeting efficiencies similar to that of TALENs. We also find that off\u2010target mutagenesis seems to be negligible, and therefore, CRISPR\/Cas may be a useful system for creating genome modifications in this important model organism. genesis 51:827\u2013834. \u00a9 2013 Wiley Periodicals, Inc.\n                  <\/jats:p>","DOI":"10.1002\/dvg.22719","type":"journal-article","created":{"date-parts":[[2013,10,12]],"date-time":"2013-10-12T11:41:04Z","timestamp":1381578064000},"page":"827-834","source":"Crossref","is-referenced-by-count":175,"title":["Biallelic genome modification in F\n                    <sub>0<\/sub>\n                    <i>Xenopus tropicalis<\/i>\n                    embryos using the CRISPR\/Cas system"],"prefix":"10.1002","volume":"51","author":[{"given":"Ira L.","family":"Blitz","sequence":"first","affiliation":[{"name":"Department of Developmental and Cell Biology University of California Irvine California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacob","family":"Biesinger","sequence":"additional","affiliation":[{"name":"Department of Computer Science University of California Irvine California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaohui","family":"Xie","sequence":"additional","affiliation":[{"name":"Department of Computer Science University of California Irvine California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ken W.Y.","family":"Cho","sequence":"additional","affiliation":[{"name":"Department of Developmental and Cell Biology University of California Irvine California"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,11,21]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2013.06.020"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-genet-110410-132430"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.21.1.289-297.2001"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.12.9.1269"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1178811"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.2507"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1231143"},{"key":"e_1_2_5_9_1","article-title":"CRISPR\/Cas9 systems targeting \u03b2\u2010globin and CCR5 genes have substantial off\u2010target activity","author":"Cradick TJ","journal-title":"Nucleic Acids 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table of Xenopus laevis (Daudin)","author":"Nieuwkoop PD","year":"1967"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2013.02.022"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkr606"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-genom-091212-153435"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1242\/bio.20133855"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2013.04.025"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-genet-110711-155447"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2013.08.022"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1102030108"},{"key":"e_1_2_5_32_1","article-title":"RNA\u2010guided genome editing in plants using a CRISPR\u2010Cas system","author":"Xie K","journal-title":"Mol 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