{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T14:20:37Z","timestamp":1791123637940,"version":"4.1.0"},"reference-count":24,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2013,11,8]],"date-time":"2013-11-08T00:00:00Z","timestamp":1383868800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genesis"],"published-print":{"date-parts":[[2013,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    We have assessed the efficacy of the recently developed CRISPR\/Cas (clustered regularly interspaced short palindromic repeats\/CRISPR\u2010associated) system for genome modification in the amphibian\n                    <jats:italic>Xenopus tropicalis<\/jats:italic>\n                    . As a model experiment, targeted mutations of the\n                    <jats:italic>tyrosinase<\/jats:italic>\n                    gene were verified, showing the expected albinism phenotype in injected embryos. We further tested this technology by interrupting the\n                    <jats:italic>six3<\/jats:italic>\n                    gene, which is required for proper eye and brain formation. Expected eye and brain phenotypes were observed when inducing mutations in the\n                    <jats:italic>six3<\/jats:italic>\n                    coding regions, as well as when deleting the gene promoter by dual targeting. We describe here a standardized protocol for genome editing using this system. This simple and fast method to edit the genome provides a powerful new reverse genetics tool for\n                    <jats:italic>Xenopus<\/jats:italic>\n                    researchers. genesis 51:835\u2013843. \u00a9 2013 Wiley Periodicals, Inc.\n                  <\/jats:p>","DOI":"10.1002\/dvg.22720","type":"journal-article","created":{"date-parts":[[2013,10,12]],"date-time":"2013-10-12T11:08:38Z","timestamp":1381576118000},"page":"835-843","source":"Crossref","is-referenced-by-count":258,"title":["Simple and efficient CRISPR\/Cas9\u2010mediated targeted mutagenesis in\n                    <i>Xenopus tropicalis<\/i>"],"prefix":"10.1002","volume":"51","author":[{"given":"Takuya","family":"Nakayama","sequence":"first","affiliation":[{"name":"Department of Biology University of Virginia Charlottesville Virginia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margaret B.","family":"Fish","sequence":"additional","affiliation":[{"name":"Department of Biology University of Virginia Charlottesville Virginia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marilyn","family":"Fisher","sequence":"additional","affiliation":[{"name":"Department of Biology University of Virginia Charlottesville Virginia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jamina","family":"Oomen\u2010Hajagos","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Cell Biology Stony Brook University Stony Brook New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerald H.","family":"Thomsen","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Cell Biology Stony Brook University Stony Brook New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert M.","family":"Grainger","sequence":"additional","affiliation":[{"name":"Department of Biology University of Virginia Charlottesville 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