{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T08:11:55Z","timestamp":1789978315813,"version":"4.0.1"},"reference-count":20,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2014,1,31]],"date-time":"2014-01-31T00:00:00Z","timestamp":1391126400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genesis"],"published-print":{"date-parts":[[2014,4]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>As a dual function protein, \u03b2\u2010catenin affects both cell adhesion and mediates canonical Wnt\/\u03b2\u2010catenin cell signaling. \u03b2\u2010Catenin is prominently expressed in somatic Sertoli cells in the testis and postmeiotic germ cells, suggesting an additional role in spermatogenesis. It was reported previously that Cre\/loxP\u2010mediated conditional inactivation of the \u03b2\u2010catenin gene (<jats:italic>Ctnnb1<\/jats:italic>) in male gonads using a protamine promoter\u2010driven Cre transgene (<jats:italic>Prm\u2010cre<\/jats:italic>) resulted in partial infertility, reduced sperm count, and abnormal spermatogenesis. In this report, we demonstrated that the conditional deletion of <jats:italic>Ctnnb1<\/jats:italic> using a germ cell specific Cre transgene (<jats:italic>Stra8\u2010icre<\/jats:italic>) had no effect on male fertility. We have shown that the <jats:italic>Stra8\u2010icre<\/jats:italic> transgene was highly efficient in generating deletion in early pre\u2010meiotic and post\u2010meiotic cells. No differences in anatomical or histological presentation were found in the mutant testis, the production of viable sperm was similar, and no abnormalities in DNA sperm content were detected. We concluded that \u03b2\u2010catenin is fully dispensable in germ cells for spermatogenesis. The conflicting results from the earlier study may have been due to off\u2010target expression of <jats:italic>Prm\u2010cre<\/jats:italic> in testicular somatic cells. In future studies, the analysis of conditional mutants using several Cre\u2010transgenes should be encouraged to reduce potential errors. genesis 52:328\u2013332, 2014. \u00a9 2014 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/dvg.22742","type":"journal-article","created":{"date-parts":[[2014,1,20]],"date-time":"2014-01-20T12:12:52Z","timestamp":1390219972000},"page":"328-332","source":"Crossref","is-referenced-by-count":16,"title":["Normal fertility in male mice with deletion of \u03b2\u2010catenin gene in germ cells"],"prefix":"10.1002","volume":"52","author":[{"given":"Bryan","family":"Rivas","sequence":"first","affiliation":[{"name":"Department of Human and Molecular Genetics Herbert Wertheim College of Medicine, Florida International University Miami Florida"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zaohua","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Human and Molecular Genetics Herbert Wertheim College of Medicine, Florida International University Miami Florida"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander I.","family":"Agoulnik","sequence":"additional","affiliation":[{"name":"Department of Human and Molecular Genetics Herbert Wertheim College of Medicine, Florida International University Miami Florida"},{"name":"Department of Obstetrics and Gynecology Baylor College of Medicine Houston Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,1,31]]},"reference":[{"key":"e_1_2_2_2_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.108.068627"},{"key":"e_1_2_2_3_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.128.8.1253"},{"key":"e_1_2_2_4_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.018572"},{"key":"e_1_2_2_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0028039"},{"key":"e_1_2_2_6_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0025641"},{"key":"e_1_2_2_7_1","doi-asserted-by":"publisher","DOI":"10.1159\/000152038"},{"key":"e_1_2_2_8_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.112.103382"},{"key":"e_1_2_2_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0071213"},{"key":"e_1_2_2_10_1","doi-asserted-by":"publisher","DOI":"10.1210\/me.2010-0330"},{"key":"e_1_2_2_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2006.06.038"},{"key":"e_1_2_2_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1673-8527(08)60107-1"},{"key":"e_1_2_2_13_1","doi-asserted-by":"publisher","DOI":"10.1080\/014850190923387"},{"key":"e_1_2_2_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddn362"},{"key":"e_1_2_2_15_1","doi-asserted-by":"publisher","DOI":"10.4161\/spmg.23181"},{"key":"e_1_2_2_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddn193"},{"key":"e_1_2_2_17_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.26.14602"},{"key":"e_1_2_2_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvg.20437"},{"key":"e_1_2_2_19_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.109.079335"},{"key":"e_1_2_2_20_1","doi-asserted-by":"publisher","DOI":"10.1210\/me.2011-0057"},{"key":"e_1_2_2_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2011.06.010"}],"container-title":["genesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fdvg.22742","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dvg.22742","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,6]],"date-time":"2023-10-06T16:30:47Z","timestamp":1696609847000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/dvg.22742"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,1,31]]},"references-count":20,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2014,4]]}},"alternative-id":["10.1002\/dvg.22742"],"URL":"https:\/\/doi.org\/10.1002\/dvg.22742","archive":["Portico"],"relation":{},"ISSN":["1526-954X","1526-968X"],"issn-type":[{"value":"1526-954X","type":"print"},{"value":"1526-968X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,1,31]]}}}