{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T04:09:26Z","timestamp":1790741366431,"version":"4.1.0"},"reference-count":53,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2014,3,17]],"date-time":"2014-03-17T00:00:00Z","timestamp":1395014400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genesis"],"published-print":{"date-parts":[[2014,6]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The first molecular herald of organ asymmetry during murine embryogenesis is found at the periphery of the node in early\u2010somite stage embryos. Asymmetric gene expression and calcium accumulation at the node occurs in response to a left\u2010ward flow of extracellular fluid across the node, generated by motile cilia within the pit of the node and likely sensed by immotile cilia in the periphery of the node. The ciliation of node cells is controlled by a cascade of node\u2010restricted transcription factor activity during mid\u2010late gastrulation. Mutation of the murine Zic2 transcription factor is associated with random cardiac situs and a loss of asymmetric gene expression at the early\u2010somite node and in the lateral plate. <jats:italic>Zic2<\/jats:italic> is not expressed in these regions but is transiently expressed in the mid\u2010late gastrula node at the time of ciliogenesis. The cilia of the node are overtly abnormal in <jats:italic>Zic2<\/jats:italic> mutant embryos being dysmorphic and short relative to wild\u2010type littermates. The expression of the <jats:italic>Noto<\/jats:italic>, <jats:italic>Rfx3<\/jats:italic>, and <jats:italic>Foxj1<\/jats:italic> transcription factors known to regulate ciliogenesis is greatly depleted in the mid\u2010gastrula node of mutants, as is the expression of the <jats:italic>Pkd1l1<\/jats:italic> gene required for cilia function. Zic2 appears to be a component of the gene regulatory network that drives ciliation of node cells during gastrulation. genesis 52:626\u2013635, 2014. \u00a9 2014 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/dvg.22767","type":"journal-article","created":{"date-parts":[[2014,3,3]],"date-time":"2014-03-03T09:37:22Z","timestamp":1393839442000},"page":"626-635","source":"Crossref","is-referenced-by-count":21,"title":["The <i>Zic2<\/i> gene directs the formation and function of node cilia to control cardiac situs"],"prefix":"10.1002","volume":"52","author":[{"given":"Kristen S.","family":"Barratt","sequence":"first","affiliation":[{"name":"Early Mammalian Development Laboratory Research School of Biology, Evolution, Ecology and Genetics, The Australian National University Canberra ACT 0200 Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hannah C.","family":"Glanville\u2010Jones","sequence":"additional","affiliation":[{"name":"Early Mammalian Development Laboratory Research School of Biology, Evolution, Ecology and Genetics, The Australian National University Canberra ACT 0200 Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruth M.","family":"Arkell","sequence":"additional","affiliation":[{"name":"Early Mammalian Development Laboratory Research School of Biology, Evolution, Ecology and Genetics, The Australian National University Canberra ACT 0200 Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,3,17]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1242\/dmm.011668"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2012.08.012"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.072728"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(94)90522-3"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00335-001-2066-2"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1098\/rsob.120030"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0704344104"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0177(199604)205:4<471::AID-AJA10>3.0.CO;2-4"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0500584102"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.24.10.4417-4427.2004"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddi037"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2012.01.011"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/381155a0"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.120.7.1919"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.051714"},{"key":"e_1_2_6_17_1","first-page":"505","article-title":"Overlapping and distinct expression domains of Zic2 and Zic3 during mouse gastrulation","volume":"4","author":"Elms P","year":"2004","journal-title":"GEP"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2003.09.005"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.21874"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.058149"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng1197-305"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceb.2012.01.002"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-013-1285-5"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.21598"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/381158a0"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.306504"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.2002.0835"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(03)00511-7"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/381151a0"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81472-5"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00006.2003"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/78140"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature00849"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81705-5"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1186\/1741-7007-10-102"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1999.9240"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(02)00869-2"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.129.9.2293"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/humu.20982"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/29004"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms1624"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.02384"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.02470"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1136\/jmg.2009.073049"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1002010309"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddt001"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1042\/BC20100035"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0045252"},{"key":"e_1_2_6_49_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.1001276"},{"key":"e_1_2_6_50_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.20719"},{"key":"e_1_2_6_51_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddn197"},{"key":"e_1_2_6_52_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.00408"},{"key":"e_1_2_6_53_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1222538"},{"key":"e_1_2_6_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2004.09.207"}],"container-title":["genesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fdvg.22767","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fdvg.22767","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dvg.22767","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,13]],"date-time":"2023-09-13T00:55:48Z","timestamp":1694566548000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/dvg.22767"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,3,17]]},"references-count":53,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2014,6]]}},"alternative-id":["10.1002\/dvg.22767"],"URL":"https:\/\/doi.org\/10.1002\/dvg.22767","archive":["Portico"],"relation":{},"ISSN":["1526-954X","1526-968X"],"issn-type":[{"value":"1526-954X","type":"print"},{"value":"1526-968X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,3,17]]}}}