{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T16:38:59Z","timestamp":1762101539597},"reference-count":32,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2014,4,15]],"date-time":"2014-04-15T00:00:00Z","timestamp":1397520000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genesis"],"published-print":{"date-parts":[[2014,5]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The <jats:italic>transforming acidic coiled\u2010coil containing protein 2<\/jats:italic> (<jats:italic>Tacc2)<\/jats:italic> gene and its paralogs, <jats:italic>Tacc1<\/jats:italic> and <jats:italic>Tacc3<\/jats:italic> encode proteins that are associated with the centrosome and involved in microtubule assembly during the cell cycle. <jats:italic>Tacc2<\/jats:italic> produces several splice variants, which are poorly characterized, especially in the rat. Characterization of the temporal\/spatial expression patterns of these isoforms would be useful in understanding their distinct and overlapping functions. By comparative sequence analyses of <jats:italic>Tacc2<\/jats:italic> in multiple species, we identified a third splice variant in rat, which is much shorter in size (1,021 aa) than the longest isoform (2,834 aa). This newly identified <jats:italic>Tacc2<\/jats:italic> splice variant (isoform 3) uses a distinct first exon and generates a different open reading frame. Although Isoform 3 is expressed predominantly during developmental stages, the long <jats:italic>Tacc2<\/jats:italic> isoform (isoform 1) is distributed mainly in adult tissues. Multiple protein sequence analyses revealed that <jats:italic>Tacc2<\/jats:italic> Isoform 3 could be the ancient form, as it is conserved in mammals, birds, and amphibians; whereas the long <jats:italic>Tacc2<\/jats:italic> isoforms may have evolved in the mammalian lineage by adding exons toward the 5\u2032 region of the ancient isoform. genesis 52:378\u2013386, 2014. \u00a9 2014 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/dvg.22776","type":"journal-article","created":{"date-parts":[[2014,4,2]],"date-time":"2014-04-02T10:18:16Z","timestamp":1396433896000},"page":"378-386","source":"Crossref","is-referenced-by-count":6,"title":["Expression pattern of an evolutionarily conserved splice variant in the rat <i>Tacc2<\/i> gene"],"prefix":"10.1002","volume":"52","author":[{"given":"Bhaja K.","family":"Padhi","sequence":"first","affiliation":[{"name":"Ophthalmology Wilmer Eye Institute, The Johns Hopkins University School of Medicine Baltimore Maryland"},{"name":"Hazard Identification Division, Environmental Health Science and Research Bureau Health Canada Ottawa Ontario Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr.","given":"J. 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