{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T21:37:59Z","timestamp":1789421879843,"version":"build-2803163510"},"reference-count":46,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2014,5,2]],"date-time":"2014-05-02T00:00:00Z","timestamp":1398988800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["NIH R01HL082098-6"],"award-info":[{"award-number":["NIH R01HL082098-6"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["NIH T32GM008539-16"],"award-info":[{"award-number":["NIH T32GM008539-16"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Tri-Institutional Stem Cell Initiative","award":["Tri-SCI #2007-026"],"award-info":[{"award-number":["Tri-SCI #2007-026"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genesis"],"published-print":{"date-parts":[[2014,7]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Epidermal growth factor\u2010like domain 7 (<jats:italic>Egfl7<\/jats:italic>) expression in the developing embryo is largely restricted to sites of mesodermal progenitors of angioblasts\/hemangioblasts and the vascular endothelium. We hypothesize that <jats:italic>Egfl7<\/jats:italic> marks the endothelial lineage during embryonic development, and can be used to define the emergence of endothelial progenitor cells, as well as to visualize newly\u2010forming vasculature in the embryo and during the processes of physiologic and pathologic angiogenesis in the adult. We have generated a transgenic mouse strain that expresses enhanced green fluorescent protein (eGFP) under the control of a minimal <jats:italic>Egfl7<\/jats:italic> regulatory sequence (Egfl7:eGFP). Expression of the transgene recapitulated that of endogenous <jats:italic>Egfl7<\/jats:italic> at sites of vasculogenesis and angiogenesis in the allantois, yolk sac, and in the embryo proper. The transgene was not expressed in the quiescent endothelium of most adult organs. However, the uterus and ovary, which undergo vascular growth and remodeling throughout the estrus cycle, expressed high levels of Egfl7:eGFP. Importantly, expression of the Egfl7:eGFP transgene was induced in adult neovasculature. We also found that increased <jats:italic>Egfl7<\/jats:italic> expression contributed to pathologic revascularization in the mouse retina. To our knowledge, this is the first mouse model that enables monitoring of endothelial cells at sites of active vasculogenesis and angiogenesis. This model also facilitated the isolation and characterization of EGFL7<jats:sup>+<\/jats:sup> endothelial cell populations by fluorescence activated cell sorting (FACS). Together, our results demonstrate that the Egfl7:eGFP reporter mouse is a valuable tool that can be used to elucidate the mechanisms by which blood vessels form during development and under pathologic circumstances. genesis 52:657\u2013670, 2014. \u00a9 2014 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/dvg.22781","type":"journal-article","created":{"date-parts":[[2014,4,17]],"date-time":"2014-04-17T08:26:43Z","timestamp":1397723203000},"page":"657-670","source":"Crossref","is-referenced-by-count":22,"title":["Epidermal growth factor\u2010like domain 7 is a marker of the endothelial lineage and active angiogenesis"],"prefix":"10.1002","volume":"52","author":[{"given":"Kathryn","family":"Bambino","sequence":"first","affiliation":[{"name":"Department of Cell and Developmental Biology Weill Cornell Medical College New York New York"},{"name":"Department of Cell and Developmental Biology, Weill Cornell Graduate School of Medical Sciences Program in Biochemistry and Structural Biology, Cell and Developmental Biology, and Molecular Biology New York New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lauretta A.","family":"Lacko","sequence":"additional","affiliation":[{"name":"Department of Cell and Developmental Biology Weill Cornell Medical College New York New York"},{"name":"Department of Cell and Developmental Biology, Weill Cornell Graduate School of Medical Sciences Program in Physiology, Biophysics, and Systems Biology New York New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katherine A.","family":"Hajjar","sequence":"additional","affiliation":[{"name":"Department of Cell and Developmental Biology Weill Cornell Medical College New York New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heidi","family":"Stuhlmann","sequence":"additional","affiliation":[{"name":"Department of Cell and Developmental Biology Weill Cornell Medical College New York New York"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,5,2]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2012-03-390070"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2011.435"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)62972-0"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/74651"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm0603-653"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature04478"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.3700277"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2013.03.003"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10456-007-9066-0"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2009.187"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddi056"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.125.22.4507"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V95.5.1671.005k39_1671_1679"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/2040-2384-2-4"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2008.07.008"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.20063"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/gene.20139"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10456-007-9065-1"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature04482"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200302047"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.jcbfm.9600408"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80108-7"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm3176"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.modgep.2006.11.002"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00432-010-0832-9"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.029736"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.20821"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0012156"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcm.2005.06.002"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2010-03-274860"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2011-10-322446"},{"key":"e_1_2_6_33_1","first-page":"115","article-title":"Growth of microvessels in serum\u2010free matrix culture of rat aorta. A quantitative assay of angiogenesis in vitro","volume":"63","author":"Nicosia RF","year":"1990","journal-title":"Lab Invest"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2011-11-394882"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature02416"},{"key":"e_1_2_6_36_1","first-page":"519","article-title":"A simple, quantitative method for assessing angiogenesis and antiangiogenic agents using reconstituted basement membrane, heparin, and fibroblast growth factor","volume":"67","author":"Passaniti A","year":"1992","journal-title":"Lab Invest"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.3.905"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0090455"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.6.3.1371260"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/386671a0"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cb.11.110195.000445"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1136\/bjo.2008.142646"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.002576"},{"key":"e_1_2_6_44_1","first-page":"101","article-title":"Oxygen\u2010induced retinopathy in the mouse","volume":"35","author":"Smith LE","year":"1994","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/cdg549"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2008.07.002"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00178.2007"}],"container-title":["genesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fdvg.22781","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dvg.22781","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,6]],"date-time":"2023-10-06T21:10:59Z","timestamp":1696626659000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/dvg.22781"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,5,2]]},"references-count":46,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2014,7]]}},"alternative-id":["10.1002\/dvg.22781"],"URL":"https:\/\/doi.org\/10.1002\/dvg.22781","archive":["Portico"],"relation":{},"ISSN":["1526-954X","1526-968X"],"issn-type":[{"value":"1526-954X","type":"print"},{"value":"1526-968X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,5,2]]}}}