{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T16:52:53Z","timestamp":1790009573671,"version":"4.0.1"},"reference-count":31,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2014,5,8]],"date-time":"2014-05-08T00:00:00Z","timestamp":1399507200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["HL107147"],"award-info":[{"award-number":["HL107147"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["HD069747"],"award-info":[{"award-number":["HD069747"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["HL007914"],"award-info":[{"award-number":["HL007914"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100001209","name":"KNIGHTS TEMPLAR EYE FOUNDATION","doi-asserted-by":"publisher","award":["-"],"award-info":[{"award-number":["-"]}],"id":[{"id":"10.13039\/100001209","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genesis"],"published-print":{"date-parts":[[2014,7]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>ADAMTS9 is the most conserved member of a large family of secreted metalloproteases having diverse functions. <jats:italic>Adamts9<\/jats:italic> null mice die before gastrulation, precluding investigations of its roles later in embryogenesis, in adult mice or disease models. We therefore generated a floxed <jats:italic>Adamts9<\/jats:italic> allele to bypass embryonic lethality. In this mutant, unidirectional loxP sites flank exons 5\u20138, which encode the catalytic domain, including the protease active site. Mice homozygous for the floxed allele were viable, lacked an overt phenotype, and were fertile. Conversely, mice homozygous for a germ\u2010line deletion produced from the floxed allele by <jats:italic>Cre<\/jats:italic>\u2010lox recombination did not survive past gastrulation. Hemizygosity of the deleted <jats:italic>Adamts9<\/jats:italic> in combination with mutant <jats:italic>Adamts20<\/jats:italic> led to cleft palate and severe white spotting as previously described. Previously, <jats:italic>Adamts9<\/jats:italic> haploinsufficiency combined with either <jats:italic>Adamts20<\/jats:italic> or <jats:italic>Adamts5<\/jats:italic> nullizygosity suggested a cooperative role in interdigital web regression, but the outcome of deletion of <jats:italic>Adamts9<\/jats:italic> alone remained unknown. Here, <jats:italic>Adamts9<\/jats:italic> was conditionally deleted in limb mesoderm using <jats:italic>Prx1<\/jats:italic>\u2010Cre mice. Unlike other ADAMTS single knockouts, limb\u2010specific <jats:italic>Adamts9<\/jats:italic> deletion resulted in soft\u2010tissue syndactyly (STS) with 100% penetrance and concurrent deletion of <jats:italic>Adamts5<\/jats:italic> increased the severity of STS. Thus, <jats:italic>Adamts9<\/jats:italic> has both non\u2010redundant and cooperative roles in ensuring interdigital web regression. This new allele will be useful for investigating other biological functions of ADAMTS9. genesis 52:702\u2013712, 2014. \u00a9 2014 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/dvg.22784","type":"journal-article","created":{"date-parts":[[2014,4,20]],"date-time":"2014-04-20T08:50:40Z","timestamp":1397983840000},"page":"702-712","source":"Crossref","is-referenced-by-count":54,"title":["A new <i>Adamts9<\/i> conditional mouse allele identifies its non\u2010redundant role in interdigital web regression"],"prefix":"10.1002","volume":"52","author":[{"given":"Johanne","family":"Dubail","sequence":"first","affiliation":[{"name":"Department of Biomedical Engineering Lerner Research Institute Cleveland Clinic Cleveland Ohio"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noriko","family":"Aramaki\u2010Hattori","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering Lerner Research Institute Cleveland Clinic Cleveland 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R.","family":"Olsen","sequence":"additional","affiliation":[{"name":"Harvard School of Dental Medicine Boston Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suneel S.","family":"Apte","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering Lerner Research Institute Cleveland Clinic Cleveland 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