{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,6]],"date-time":"2023-10-06T06:11:10Z","timestamp":1696572670823},"reference-count":37,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2014,8,8]],"date-time":"2014-08-08T00:00:00Z","timestamp":1407456000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"VIB Versalius Research Center- Katholieke Universiteit Leuven"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genesis"],"published-print":{"date-parts":[[2014,10]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The Down syndrome cell adhesion molecule (<jats:italic>DSCAM<\/jats:italic>) is an Ig containing cell adhesion molecule with remarkable structural conservation throughout metazoans. In insects, <jats:italic>DSCAM<\/jats:italic> has 38,000 potential isoforms that convey axon guidance, fasciculation, and dendrite morphogenesis during neurodevelopment. In vertebrates, <jats:italic>DSCAM<\/jats:italic> is expressed throughout the nervous system and seems to also mediate proper axonal guidance and synaptogenesis without the isoform diversity found in insects. Differences in <jats:italic>DSCAM<\/jats:italic> function among several vertebrate species complicate the understanding of an evolutionarily conserved role during embryogenesis. We take advantage of the frog developmental model <jats:italic>Xenopus tropicalis<\/jats:italic> to study <jats:italic>DSCAM<\/jats:italic> function in early development by expression analysis and morpholino\u2010mediated knockdown. Our results indicate that <jats:italic>DSCAM<\/jats:italic> is expressed early in development and restricted to the head and nervous system. Knockdown of protein expression results in early morphogenetic phenotypes characterized by failed gastrulation and improper posterior neural tube closure. Our results reveal a specific, fundamental role of <jats:italic>DSCAM<\/jats:italic> in early morphogenetic movements, presumably through its well\u2010known role in homophilic cell adhesion. genesis 52:849\u2013857, 2014. \u00a9 2014 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/dvg.22804","type":"journal-article","created":{"date-parts":[[2014,8,1]],"date-time":"2014-08-01T21:31:39Z","timestamp":1406928699000},"page":"849-857","source":"Crossref","is-referenced-by-count":4,"title":["Down syndrome cell adhesion molecule is important for early development in <scp><i>X<\/i><\/scp><i>enopus tropicalis<\/i>"],"prefix":"10.1002","volume":"52","author":[{"given":"Heidi D.","family":"Morales Diaz","sequence":"first","affiliation":[{"name":"Department of Molecular, Cellular and Developmental Biology University of California Santa Barbara, California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,8,8]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3624-08.2009"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/00125817-200103000-00002"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1159\/000048808"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(02)02548-2"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.6015-10.2011"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2006.03.034"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1159\/000472398"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/441582a"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2004.11.012"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature06514"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2009.09.027"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.conb.2009.07.013"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvg.20662"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-679X(08)60307-6"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1183670"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1486806"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2007.04.013"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.10184"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.11.4997"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.06128-11"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature13200"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2008.05.030"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2007.04.013"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature06147"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0955-0674(99)00039-3"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0b013e318206b67b"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm1285"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1098992"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1242\/dmm.012138"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1159\/000203129"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1101\/pdb.prot5293"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2007.03.029"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2003.10.024"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.15.6751"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/7.2.227"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature06469"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2004.12.001"}],"container-title":["genesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fdvg.22804","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dvg.22804","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,5]],"date-time":"2023-10-05T20:57:01Z","timestamp":1696539421000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/dvg.22804"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,8,8]]},"references-count":37,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2014,10]]}},"alternative-id":["10.1002\/dvg.22804"],"URL":"https:\/\/doi.org\/10.1002\/dvg.22804","archive":["Portico"],"relation":{},"ISSN":["1526-954X","1526-968X"],"issn-type":[{"value":"1526-954X","type":"print"},{"value":"1526-968X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,8,8]]}}}