{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T20:39:39Z","timestamp":1778186379163,"version":"3.51.4"},"reference-count":38,"publisher":"Wiley","issue":"3-4","license":[{"start":{"date-parts":[[2015,4,13]],"date-time":"2015-04-13T00:00:00Z","timestamp":1428883200000},"content-version":"vor","delay-in-days":43,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["F32DE021920"],"award-info":[{"award-number":["F32DE021920"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01DE017699"],"award-info":[{"award-number":["R01DE017699"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["P30NS048154"],"award-info":[{"award-number":["P30NS048154"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genesis"],"published-print":{"date-parts":[[2015,3]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Cranial neural crest cells are specified and migrate into the pharyngeal arches where they subsequently interact with the surrounding environment. Signaling and transcription factors, such as <jats:italic>prdm1a<\/jats:italic> regulate this interaction, but it remains unclear which specific factors are required for posterior pharyngeal arch development. Previous analysis suggests that <jats:italic>prdm1a<\/jats:italic> is required for posterior ceratobranchial cartilages in zebrafish and microarray analysis between wildtype and <jats:italic>prdm1a<\/jats:italic> mutants at 25 h post fertilization demonstrated that integrin \u03b15 (<jats:italic>itga5)<\/jats:italic> is differentially expressed in <jats:italic>prdm1a<\/jats:italic> mutants. Here, we further investigate the interaction between <jats:italic>prdm1a<\/jats:italic> and <jats:italic>itga5<\/jats:italic> in zebrafish craniofacial development. In situ hybridization for <jats:italic>itga5<\/jats:italic> demonstrates that expression of <jats:italic>itga5<\/jats:italic> is decreased in <jats:italic>prdm1a<\/jats:italic> mutants between 18 and 31 h post fertilization and <jats:italic>itga5<\/jats:italic> expression overlaps with <jats:italic>prdm1a<\/jats:italic> in the posterior arches, suggesting a temporal window for interaction. Double mutants for <jats:italic>prdm1a;itga5<\/jats:italic> have an additive viscerocranium phenotype more similar to <jats:italic>prdm1a<\/jats:italic> mutants, suggesting that <jats:italic>prdm1a<\/jats:italic> acts upstream of <jats:italic>itga5<\/jats:italic>. Consistent with this, loss of posterior pharyngeal arch expression of <jats:italic>dlx2a<\/jats:italic>, ceratobranchial cartilages 2\u20135, and cell proliferation in <jats:italic>prdm1a<\/jats:italic> mutants can be rescued with <jats:italic>itga5<\/jats:italic> mRNA injection. Taken together, these data suggest that <jats:italic>prdm1a<\/jats:italic> acts upstream of <jats:italic>itga5<\/jats:italic> and are both necessary for posterior pharyngeal arch development in zebrafish. genesis 53:270\u2013277, 2015. \u00a9 2015 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/dvg.22850","type":"journal-article","created":{"date-parts":[[2015,3,25]],"date-time":"2015-03-25T08:43:04Z","timestamp":1427272984000},"page":"270-277","source":"Crossref","is-referenced-by-count":5,"title":["<i>prdm1a<\/i> functions upstream of <i>itga5<\/i> in zebrafish craniofacial development"],"prefix":"10.1002","volume":"53","author":[{"given":"Kristi","family":"LaMonica","sequence":"first","affiliation":[{"name":"Department of Craniofacial Biology School of Dental Medicine University of Colorado Aurora Colrado"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hai\u2010lei","family":"Ding","sequence":"additional","affiliation":[{"name":"Department of Craniofacial Biology School of Dental Medicine University of Colorado Aurora Colrado"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kristin Bruk","family":"Artinger","sequence":"additional","affiliation":[{"name":"Department of Craniofacial Biology School of Dental Medicine University of Colorado Aurora Colrado"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,4,13]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.14-06-03475.1994"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.126.18.3969"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0925-4773(00)00518-9"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0027778"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.22090"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.21439"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.20833"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/bdrc.20007"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1999.9527"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.0020244"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2013.08.011"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0024443"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.02281"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.015719"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.24181"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajmg.a.10980"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0812334106"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.018093"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2004.11.014"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2011.06.034"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.038935"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1002030302"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.20905"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2005.03.006"},{"key":"e_1_2_5_26_1","volume-title":"The 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