{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T13:45:04Z","timestamp":1762004704263,"version":"3.37.3"},"reference-count":10,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2015,6,11]],"date-time":"2015-06-11T00:00:00Z","timestamp":1433980800000},"content-version":"vor","delay-in-days":10,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology of Japan","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genesis"],"published-print":{"date-parts":[[2015,6]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>A mammalian body is composed of more than 200 different types of cells. The purification of a certain cell type from tissues\/organs enables a wide variety of studies. One popular cell purification method is immunological isolation, using antibodies against specific cell surface antigens. However, this is not a general\u2010purpose method, since suitable antigens have not been found in certain cell types, including embryonic gonadal somatic cells and Sertoli cells. To address this issue, we established a knock\u2010in mouse line, named <jats:italic>R26 KI<\/jats:italic>, designed to express the human cell surface antigen hCD271 through Cre\/loxP\u2010mediated recombination. First, we used the <jats:italic>R26 Kl<\/jats:italic> mouse line to purify embryonic gonadal somatic cells. Gonadal somatic cells were purified from the <jats:italic>R26 KI<\/jats:italic>; <jats:italic>Nr5a1\u2010Cre<\/jats:italic>\u2010transgenic (tg) embryos almost equally as efficiently as from <jats:italic>Nr5a1\u2010hCD271<\/jats:italic>\u2010tg embryos. Second, we used the <jats:italic>R26 KI<\/jats:italic> mouse line to purify Sertoli cells successfully from <jats:italic>R26 KI<\/jats:italic>; <jats:italic>Amh\u2010Cre<\/jats:italic>\u2010tg testes. In summary, we propose that the <jats:italic>R26 KI<\/jats:italic> mouse line is a powerful tool for the purification of various cell types. genesis 53:387\u2013393, 2015. \u00a9 2015 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/dvg.22863","type":"journal-article","created":{"date-parts":[[2015,5,27]],"date-time":"2015-05-27T00:10:04Z","timestamp":1432685404000},"page":"387-393","source":"Crossref","is-referenced-by-count":9,"title":["Development of a general\u2010purpose method for cell purification using <scp>C<\/scp>re\/lox<scp>P<\/scp>\u2010mediated recombination"],"prefix":"10.1002","volume":"53","author":[{"given":"Shunsuke","family":"Kuroki","sequence":"first","affiliation":[{"name":"Department of Enzyme Chemistry Institute for Enzyme Research, Tokushima University Tokushima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mika","family":"Akiyoshi","sequence":"additional","affiliation":[{"name":"Experimental Research Center for Infectious Diseases, Institute for Virus Research Kyoto University Sakyo\u2010Ku, Kyoto Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ko","family":"Ideguchi","sequence":"additional","affiliation":[{"name":"Department of Enzyme Chemistry Institute for Enzyme Research, Tokushima University Tokushima Japan"},{"name":"Graduate School of Biostudies Kyoto University Sakyo\u2010Ku, Kyoto Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Satsuki","family":"Kitano","sequence":"additional","affiliation":[{"name":"Experimental Research Center for Infectious Diseases, Institute for Virus Research Kyoto University Sakyo\u2010Ku, Kyoto Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hitoshi","family":"Miyachi","sequence":"additional","affiliation":[{"name":"Experimental Research Center for Infectious Diseases, Institute for Virus Research Kyoto University Sakyo\u2010Ku, Kyoto Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michiko","family":"Hirose","sequence":"additional","affiliation":[{"name":"Bioresource Center, RIKEN Tsukuba Institute Tsukuba Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nathan","family":"Mise","sequence":"additional","affiliation":[{"name":"Bioresource Center, RIKEN Tsukuba Institute Tsukuba Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kuniya","family":"Abe","sequence":"additional","affiliation":[{"name":"Bioresource Center, RIKEN Tsukuba Institute Tsukuba Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Atsuo","family":"Ogura","sequence":"additional","affiliation":[{"name":"Bioresource Center, RIKEN Tsukuba Institute Tsukuba Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Makoto","family":"Tachibana","sequence":"additional","affiliation":[{"name":"Department of Enzyme Chemistry Institute for Enzyme Research, Tokushima University Tokushima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,6,11]]},"reference":[{"key":"e_1_2_4_2_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.102.008045"},{"key":"e_1_2_4_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.3008331"},{"key":"e_1_2_4_4_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.120.10.2787"},{"key":"e_1_2_4_5_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.00957"},{"key":"e_1_2_4_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(86)90619-7"},{"key":"e_1_2_4_7_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.122.9.2813"},{"key":"e_1_2_4_8_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1239864"},{"key":"e_1_2_4_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(90)90056-L"},{"key":"e_1_2_4_10_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1164594"},{"key":"e_1_2_4_11_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.228080.113"}],"container-title":["genesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fdvg.22863","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dvg.22863","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,23]],"date-time":"2023-09-23T21:59:04Z","timestamp":1695506344000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/dvg.22863"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,6]]},"references-count":10,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2015,6]]}},"alternative-id":["10.1002\/dvg.22863"],"URL":"https:\/\/doi.org\/10.1002\/dvg.22863","archive":["Portico"],"relation":{},"ISSN":["1526-954X","1526-968X"],"issn-type":[{"type":"print","value":"1526-954X"},{"type":"electronic","value":"1526-968X"}],"subject":[],"published":{"date-parts":[[2015,6]]}}}