{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T02:33:11Z","timestamp":1790389991199,"version":"4.1.0"},"reference-count":57,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2016,1,12]],"date-time":"2016-01-12T00:00:00Z","timestamp":1452556800000},"content-version":"vor","delay-in-days":11,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National institutes of Health","doi-asserted-by":"publisher","award":["1 R15 HD068176\u201001A1"],"award-info":[{"award-number":["1 R15 HD068176\u201001A1"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genesis"],"published-print":{"date-parts":[[2016,1]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Zebrafish with defective Nodal signaling have a phenotype analogous to the fatal human birth defect anencephaly, which is caused by an open anterior neural tube. Previous work in our laboratory found that anterior open neural tube phenotypes in Nodal signaling mutants were caused by lack of mesendodermal\/mesodermal tissues. Defects in these mutants are already apparent at neural plate stage, before the neuroepithelium starts to fold into a tube. Consistent with this, we found that the requirement for Nodal signaling maps to mid\u2010late blastula stages. This timing correlates with the timing of prechordal plate mesendoderm and anterior mesoderm induction, suggesting these tissues act to promote neurulation. To further identify tissues important for neurulation, we took advantage of the variable phenotypes in Nodal signaling\u2010deficient <jats:italic>sqt<\/jats:italic> mutant and Lefty1<jats:italic>\u2010<\/jats:italic>overexpressing embryos. Statistical analysis indicated a strong, positive correlation between a closed neural tube and presence of several mesendoderm\/mesoderm\u2010derived tissues (hatching glands, cephalic paraxial mesoderm, notochord, and head muscles). However, the neural tube was closed in a subset of embryos that lacked any one of these tissues. This suggests that several types of Nodal\u2010induced mesendodermal\/mesodermal precursors are competent to promote neurulation. genesis 54:3\u201318, 2016. \u00a9 2016 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/dvg.22908","type":"journal-article","created":{"date-parts":[[2015,11,3]],"date-time":"2015-11-03T23:39:08Z","timestamp":1446593948000},"page":"3-18","source":"Crossref","is-referenced-by-count":19,"title":["Temporal and spatial requirements for Nodal\u2010induced anterior mesendoderm and mesoderm in anterior neurulation"],"prefix":"10.1002","volume":"54","author":[{"given":"Ngawang","family":"Gonsar","sequence":"first","affiliation":[{"name":"Department of Biology University of Minnesota Duluth Duluth MN"},{"name":"Integrated Biosciences Graduate Program, University of Minnesota Duluth MN"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alicia","family":"Coughlin","sequence":"additional","affiliation":[{"name":"Department of Biology University of 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