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ESR2 is one of two classical nuclear estrogen receptors and displays a spatiotemporal expression pattern and functions that are different from the other estrogen receptor, ESR1. A cassette was constructed that contained iCre, a polyadenylation sequence, and a neomycin selection marker. This construct was used to insert iCre in front of the endogenous start codon of the<jats:italic>Esr2<\/jats:italic>gene of a C57BL\/6J embryonic stem cell line via homologous recombination. Resulting Esr2\u2010iCre mice were bred with ROSA26\u2010lacZ and Ai9\u2010RFP reporter mice to visualize cells of functional iCre expression. Strong expression was observed in the ovary, the pituitary, the interstitium of the testes, the head and tail but not body of the epididymis, skeletal muscle, the coagulation gland (anterior prostate), the lung, and the preputial gland. Additional diffuse or patchy expression was observed in the cerebrum, the hypothalamus, the heart, the adrenal gland, the colon, the bladder, and the pads of the paws. Overall, Esr2\u2010iCre mice will serve as a novel line for conditionally ablating genes in<jats:italic>Esr2<\/jats:italic>\u2010expressing tissues, identifying novel<jats:italic>Esr2<\/jats:italic>\u2010expressing cells, and differentiating the functions of ESR2 and ESR1. genesis 54:38\u201352, 2016. \u00a9 2016 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/dvg.22911","type":"journal-article","created":{"date-parts":[[2015,12,10]],"date-time":"2015-12-10T05:45:01Z","timestamp":1449726301000},"page":"38-52","source":"Crossref","is-referenced-by-count":21,"title":["Generation of an estrogen receptor beta\u2010iCre knock\u2010in mouse"],"prefix":"10.1002","volume":"54","author":[{"given":"Joseph A.","family":"Cacioppo","sequence":"first","affiliation":[{"name":"Comparative Biosciences, College of Veterinary Medicine, University of Illinois Urbana\u2010Champaign Illinois 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