{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,5,1]],"date-time":"2024-05-01T14:56:17Z","timestamp":1714575377574},"reference-count":25,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2016,5,13]],"date-time":"2016-05-13T00:00:00Z","timestamp":1463097600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Grant-in-Aid for Scientific Research","award":["15K14359"],"award-info":[{"award-number":["15K14359"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genesis"],"published-print":{"date-parts":[[2016,7]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Spermatogenesis is a complex and highly regulated process by which spermatogonial stem cells differentiate into spermatozoa. To better understand the molecular mechanisms of the process, the Cre\/loxP system has been widely utilized for conditional gene knockout in mice. In this study, we generated a transgenic mouse line that expresses Cre recombinase under the control of the 2.5 kbp of the <jats:italic>Prolactin family 3, subfamily b, member 1<\/jats:italic> (<jats:italic>Prl3b1<\/jats:italic>) gene promoter (<jats:italic>Prl3b1<\/jats:italic>\u2010cre). <jats:italic>Prl3b1<\/jats:italic> was initially reported to code for placental lactogen 2 (PL\u20102) protein in placenta along with increased expression toward the end of pregnancy. PL\u20102 was found to be expressed in germ cells in the testis, especially in spermatocytes. To analyze the specificity and efficiency of Cre recombinase activity in <jats:italic>Prl3b1<\/jats:italic>\u2010cre mice, the mice were mated with reporter R26GRR mice, which express GFP ubiquitously before and tdsRed exclusively after Cre recombination. The systemic examination of <jats:italic>Prl3b1<\/jats:italic>\u2010cre;R26GRR mice revealed that tdsRed\u2010positive cells were detected only in the testis and epididymis. Fluorescence imaging of <jats:italic>Prl3b1<\/jats:italic>\u2010cre;R26GRR testes suggested that Cre\u2010mediated recombination took place in the germ cells with approximately 74% efficiency determined by <jats:italic>in vitro<\/jats:italic> fertilization. In conclusion, our results suggest that the <jats:italic>Prl3b1<\/jats:italic>\u2010<jats:italic>cre<\/jats:italic> mice line provides a unique resource to understand testicular germ\u2010cell development. genesis 54:389\u2013397, 2016. \u00a9 2016 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/dvg.22944","type":"journal-article","created":{"date-parts":[[2016,4,30]],"date-time":"2016-04-30T09:19:29Z","timestamp":1462007969000},"page":"389-397","source":"Crossref","is-referenced-by-count":5,"title":["Germline recombination in a novel Cre transgenic line, <i>Prl3b1<\/i>\u2010Cre mouse"],"prefix":"10.1002","volume":"54","author":[{"given":"Al\u2010Sayed","family":"Al\u2010Soudy","sequence":"first","affiliation":[{"name":"Laboratory Animal Resource Center University of Tsukuba Tsukuba Ibaraki Japan"},{"name":"Animal Genetic Resource Department National Gene Bank Giza Egypt"},{"name":"Department of Anatomy and Embryology, Graduate School of Comprehensive Human Sciences University of Tsukuba Tsukuba Ibaraki Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tsuyoshi","family":"Nakanishi","sequence":"additional","affiliation":[{"name":"Laboratory of Hygienic Chemistry and Molecular Toxicology Gifu Pharmaceutical University Gifu Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seiya","family":"Mizuno","sequence":"additional","affiliation":[{"name":"Laboratory Animal Resource Center University of Tsukuba Tsukuba Ibaraki Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshikazu","family":"Hasegawa","sequence":"additional","affiliation":[{"name":"Laboratory Animal Resource Center University of Tsukuba Tsukuba Ibaraki Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hossam H.","family":"Shawki","sequence":"additional","affiliation":[{"name":"Laboratory Animal Resource Center University of Tsukuba Tsukuba Ibaraki Japan"},{"name":"Animal Genetic Resource Department National Gene Bank Giza Egypt"},{"name":"Department of Anatomy and Embryology, Graduate School of Comprehensive Human Sciences University of Tsukuba Tsukuba Ibaraki Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Megumi C.","family":"Katoh","sequence":"additional","affiliation":[{"name":"Laboratory Animal Resource Center University of Tsukuba Tsukuba Ibaraki Japan"},{"name":"Department of Anatomy and Embryology, Graduate School of Comprehensive Human Sciences University of Tsukuba Tsukuba Ibaraki Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Walaa A.","family":"Basha","sequence":"additional","affiliation":[{"name":"Laboratory Animal Resource Center University of Tsukuba Tsukuba Ibaraki Japan"},{"name":"Department of Anatomy and Embryology, Graduate School of Comprehensive Human Sciences University of Tsukuba Tsukuba Ibaraki Japan"},{"name":"Department of Anatomy and Embryology, Faculty of Veterinary Medicine Suez Canal University Ismailia Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abdelaziz E.","family":"Ibrahim","sequence":"additional","affiliation":[{"name":"Laboratory Animal Resource Center University of Tsukuba Tsukuba Ibaraki Japan"},{"name":"Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine Suez Canal University Ismailia Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hany A.","family":"El\u2010Shemy","sequence":"additional","affiliation":[{"name":"Cairo University Research Park, Faculty of Agriculture, Cairo University Giza Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroyoshi","family":"Iseki","sequence":"additional","affiliation":[{"name":"Laboratory Animal Resource Center University of Tsukuba Tsukuba Ibaraki Japan"},{"name":"International Institute for Integrative Sleep Medicine (WPI\u2010IIIS), University of Tsukuba Tsukuba Ibaraki 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