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Using CrispR\/Cas9\u2010mediated homologous directed repair (HDR), we generated a new <jats:italic>Pax9\u2010CreER<\/jats:italic> knock\u2010in mouse line in which the CreER<jats:sup>T2<\/jats:sup> fusion protein is produced after synthesis of endogenous Pax9 protein. We found that tdTomato reporter expression in <jats:italic>Pax9\u2010CreER;tdTomato<\/jats:italic> reporter mice is detectable in a similar pattern to the endogenous <jats:italic>Pax9<\/jats:italic> expression, faithfully recapitulating the <jats:italic>Pax9<\/jats:italic> expression domains throughout the embryo and in the adult mouse. At early embryonic stages, the tdTomato reporter is expressed first in the pharyngeal pouch region and later in the craniofacial mesenchyme, somites, limbs, and lingual papillae in the adult tongue. These results demonstrate that this new <jats:italic>Pax9\u2010CreER<\/jats:italic> knock\u2010in mouse line can be used for lineage tracing and genetic targeting of <jats:italic>Pax9<\/jats:italic>\u2010expressing cells and their progeny in a temporally and spatially controlled manner during development and organogenesis.<\/jats:p>","DOI":"10.1002\/dvg.22956","type":"journal-article","created":{"date-parts":[[2016,7,6]],"date-time":"2016-07-06T08:36:59Z","timestamp":1467794219000},"page":"490-496","source":"Crossref","is-referenced-by-count":13,"title":["Generation and characterization of tamoxifen\u2010inducible <i>Pax9\u2010CreER<\/i> knock\u2010in mice using CrispR\/Cas9"],"prefix":"10.1002","volume":"54","author":[{"given":"Jifan","family":"Feng","sequence":"first","affiliation":[{"name":"Center for Craniofacial Molecular Biology, University of Southern California Los Angeles CA 90033 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junjun","family":"Jing","sequence":"additional","affiliation":[{"name":"Center for Craniofacial Molecular Biology, University of Southern California Los Angeles CA 90033 USA"},{"name":"State Key Laboratory of Oral Diseases West China Hospital of Stomatology, Sichuan University Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pedro A.","family":"Sanchez\u2010Lara","sequence":"additional","affiliation":[{"name":"Center for Craniofacial Molecular Biology, University of Southern California Los Angeles CA 90033 USA"},{"name":"Center for Personalized Medicine, Children's Hospital Los Angeles Los Angeles CA 90027 USA"},{"name":"Department of Pathology &amp; 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