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Otoconia abnormalities and degeneration can cause or facilitate crystal dislocation to the ampulla, leading to vertigo and imbalance in humans. In order to better understand the molecular mechanism controlling otoconia formation and maintenance, we have examined the spatial and temporal expression differences of otoconial genes in the mouse inner ear at developmental, mature and aging stages using whole transcriptome sequencing (RNA\u2010Seq) and quantitative RT\u2010PCR. We show that the expression levels of most otoconial genes are much higher in the utricle and saccule compared with other inner ear tissues before postnatal stages in C57Bl\/6J mice, and the expression of a few of these genes is restricted to the embryonic utricle and saccule. After the early postnatal stages, expression of all otoconial genes in the utricle and saccule is drastically reduced, while a few genes gain expression dominance in the aging ampulla, indicating a potential for ectopic debris formation in the latter tissue at old ages. The data suggest that the expression of otoconial genes is tightly regulated spatially and temporally during developmental stages and can become unregulated at aging stages. Birth Defects Research (Part A) 106:613\u2013625, 2016. \u00a9 2016 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/dvg.22990","type":"journal-article","created":{"date-parts":[[2016,11,16]],"date-time":"2016-11-16T13:13:56Z","timestamp":1479302036000},"page":"613-625","source":"Crossref","is-referenced-by-count":12,"title":["Spatiotemporal differences in otoconial gene expression"],"prefix":"10.1002","volume":"54","author":[{"given":"Yinfang","family":"Xu","sequence":"first","affiliation":[{"name":"Vestibular Genetics Laboratory Center for Sensory Neuroscience, Boys Town National Research Hospital Omaha Nebraska 68131 USA"},{"name":"Cell Electrophysiology Laboratory, Shanghai Research Center of Acupuncture and Meridians Shanghai 201203 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Vestibular Genetics Laboratory Center for Sensory Neuroscience, Boys Town National Research Hospital Omaha Nebraska 68131 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yunxia Wang","family":"Lundberg","sequence":"additional","affiliation":[{"name":"Vestibular Genetics Laboratory Center for Sensory Neuroscience, Boys Town National Research Hospital Omaha Nebraska 68131 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,11,12]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/cm.21227"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heares.2012.07.003"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0196-0709(80)80021-4"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.3109\/00016488809106427"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.3109\/00016488409130990"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.37.3.371"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M403046200"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1097\/MAO.0b013e318271c48b"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0012765"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.120188"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-5955(01)00312-4"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/10.2.153"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.3109\/00016488809125130"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005537-200004000-00022"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng1197-292"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005537-199705000-00010"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0705182104"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddg087"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.3109\/00016489309128117"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heares.2004.03.019"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.mlg.0000217238.84401.03"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-5955(99)00090-8"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heares.2004.01.008"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1080\/000164800750000603"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.00525.2010"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2005.12.025"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.30.18693"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)00102-1"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1006\/scdb.1997.0145"},{"key":"e_1_2_5_31_1","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1177\/00034894840930S404","article-title":"Otoconia in health and disease. 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